+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Proteomic analysis of 'Zaosu' pear (Pyrus bretschneideri Rehd.) and its early-maturing bud sport



Proteomic analysis of 'Zaosu' pear (Pyrus bretschneideri Rehd.) and its early-maturing bud sport



Plant Science 224: 120-135



Maturation of fruits involves a series of physiological, biochemical, and organoleptic changes that eventually make fleshy fruits attractive, palatable, and nutritional. In order to understand the mature mechanism of the early-maturing bud sport of 'Zaosu' pear, we analyzed the differences of proteome expression between the both pears in different mature stages by the methods of a combination of two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Seventy-five differential expressed protein spots (p<0.05) were obtained between 'Zaosu' pear and its early-maturing bud sport, but only sixty-eight were demonstratively identified in the database of NCBI and uniprot. The majority of proteins were linked to metabolism, energy, stress response/defense and cell structure. Additionally, our data confirmed an increase of proteins related to cell-wall modification, oxidative stress and pentose phosphate metabolism and a decrease of proteins related to photosynthesis and glycolysis during the development process of both pears, but all these proteins increased or decreased faster in the early-maturing bud sport. This comparative analysis between both pears showed that these proteins were closely associated with maturation and could provide more detailed characteristics of the maturation process of both pears.

(PDF emailed within 0-6 h: $19.90)

Accession: 055255636

Download citation: RISBibTeXText

PMID: 24908513

DOI: 10.1016/j.plantsci.2014.04.012



Related references

Proteomic analysis of 'Zaosu' pear (Pyrus bretschneideri Rehd.) and its red skin bud mutation. Proteome Science 10(1): 51, 2012

Use of thiamine for controlling Alternaria alternata postharvest rot in Asian pear Pyrus bretschneideri Rehd cv Zaosu. International Journal of Food Science & Technology 47(10), 2012

Characterization and expression analysis of PbEXP genes in the epidermis of pear (Pyrus bretschneideri Rehd.). Plant Growth Regulation 84(1): 1-9, 2018

Molecular cloning and expression analysis of a gene for sucrose transporter from pear (Pyrus bretschneideri Rehd.) fruit. Plant Physiology and Biochemistry 73: 63-69, 2014

Molecular analysis of two Chinese pear (Pyrus bretschneideri Rehd.) spontaneous self-compatible mutants, Yan Zhuang and Jin Zhui. Plant Biology 11(5): 774-783, 2009

The genome of the pear (Pyrus bretschneideri Rehd.). Genome Research 23(2): 396-408, 2013

Comparison of the transcriptomic analysis between two Chinese white pear (Pyrus bretschneideri Rehd.) genotypes of different stone cells contents. Plos One 12(10): E0187114, 2017

Characterization of CIPK Family in Asian Pear (Pyrus bretschneideri Rehd) and Co-expression Analysis Related to Salt and Osmotic Stress Responses. Frontiers in Plant Science 7: 1361, 2016

Genome-Wide Function, Evolutionary Characterization and Expression Analysis of Sugar Transporter Family Genes in Pear (Pyrus bretschneideri Rehd). Plant and Cell Physiology 56(9): 1721-1737, 2016

Molecular analysis of the processes of surface brown spot (SBS) formation in pear fruit (Pyrus bretschneideri Rehd. cv. Dangshansuli) by de novo transcriptome assembly. Plos One 8(9): E74217, 2014

Characterization and Expression Profiling Analysis of Calmodulin Genes in Response to Salt and Osmotic Stresses in Pear (Pyrus bretschneideri Rehd.) and in Comparison with Arabidopsis. Biomed Research International: 7904162, 2017

Methyl salicylate delays peel yellowing of 'Zaosu' pear (Pyrus bretschneideri) during storage by regulating chlorophyll metabolism and maintaining chloroplast ultrastructure. Journal of the Science of Food and Agriculture 2019, 2019

A study on the development of calyx of korla fragrant pear (Pyrus bretschneideri Rehd). Xinjiang Agricultural Sciences 44(3): 377-381, 2007

Two MYB transcription factors regulate flavonoid biosynthesis in pear fruit (Pyrus bretschneideri Rehd.). Journal of Experimental Botany 67(5): 1275-1284, 2016

Self-compatible pear cultivar 'Yanzhuang' resulting from S-RNase mutation of 'Ya Li' (Pyrus bretschneideri Rehd.). 2008