+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Proton imaging of siloxanes to map tissue oxygenation levels (PISTOL): a tool for quantitative tissue oximetry

Proton imaging of siloxanes to map tissue oxygenation levels (PISTOL): a tool for quantitative tissue oximetry

Nmr in Biomedicine 21(8): 899-907

Hexamethyldisiloxane (HMDSO) has been identified as a sensitive proton NMR indicator of tissue oxygenation (pO(2)) based on spectroscopic spin-lattice relaxometry. A rapid MRI approach has now been designed, implemented, and tested. The technique, proton imaging of siloxanes to map tissue oxygenation levels (PISTOL), utilizes frequency-selective excitation of the HMDSO resonance and chemical-shift selective suppression of residual water signal to effectively eliminate water and fat signals and pulse-burst saturation recovery (1)H echo planar imaging to map T(1) of HMDSO and hence pO(2). PISTOL was used here to obtain maps of pO(2) in rat thigh muscle and Dunning prostate R3327 MAT-Lu tumor-implanted rats. Measurements were repeated to assess baseline stability and response to breathing of hyperoxic gas. Each pO(2) map was obtained in 3(1/2) min, facilitating dynamic measurements of response to oxygen intervention. Altering the inhaled gas to oxygen produced a significant increase in mean pO(2) from 55 Torr to 238 Torr in thigh muscle and a smaller, but significant, increase in mean pO(2) from 17 Torr to 78 Torr in MAT-Lu tumors. Thus, PISTOL enabled mapping of tissue pO(2) at multiple locations and dynamic changes in pO(2) in response to intervention. This new method offers a potentially valuable new tool to image pO(2) in vivo for any healthy or diseased state by (1)H MRI.

(PDF emailed within 0-6 h: $19.90)

Accession: 055260087

Download citation: RISBibTeXText

PMID: 18574806

DOI: 10.1002/nbm.1279

Related references

A faster PISTOL for 1 H MR-based quantitative tissue oximetry. Nmr in Biomedicine 2019: E4076, 2019

Imaging acute ischemic tissue acidosis with pH-sensitive endogenous amide proton transfer (APT) MRI--correction of tissue relaxation and concomitant RF irradiation effects toward mapping quantitative cerebral tissue pH. Neuroimage 60(1): 1-6, 2012

Importance of real-time tissue oximetry: relationship to muscle oxygenation and tissue viability. Journal of Surgical Research 169(1): 156-161, 2011

Quantitative analysis of mucosal oxygenation using ex vivo imaging of healthy and inflamed mammalian colon tissue. Cellular and Molecular Life Sciences 74(1): 141-151, 2016

Quantitative brain tissue oximetry, phase spectroscopy and imaging the range of homeostasis in piglet brain. Advances in Experimental Medicine and Biology 530: 13-18, 2003

Nibs tissue oxygenation compared to pulse oximetry in critically ill patients. 2007

Evaluation of right ventricular function by quantitative tissue velocity imaging and tissue tracking imaging in neonates with congenital hypothyroidism. Zhonghua Er Ke Za Zhi 45(8): 599-603, 2007

Retrievable micro-inserts containing oxygen sensors for monitoring tissue oxygenation using EPR oximetry. Physiological Measurement 29(11): 1247-1254, 2008

Comparison of regional skeletal muscle tissue oxygenation in college athletes and sedentary control subjects using quantitative BOLD MR imaging. Physiological Reports 4(16), 2017

A semiautomatic cell counting tool for quantitative imaging of tissue engineering scaffolds. Tissue Engineering. Part C, Methods 19(9): 697-707, 2014

Effects of intermittent electrical stimulation on superficial pressure, tissue oxygenation, and discomfort levels for the prevention of deep tissue injury. Annals of Biomedical Engineering 39(2): 649-663, 2011

Real-time, in vivo determination of dynamic changes in lung and heart tissue oxygenation using EPR oximetry. Advances in Experimental Medicine and Biology 812: 81-86, 2014

In vivo evidence of methamphetamine induced attenuation of brain tissue oxygenation as measured by EPR oximetry. Toxicology and Applied Pharmacology 275(2): 73-78, 2014

Increased inspired oxygen concentration as a factor in improved brain tissue oxygenation and tissue lactate levels after severe human head injury. Journal of Neurosurgery 91(1): 1-10, 1999

Molecular and morphometric description of adipose tissue during weight changes: a quantitative tool for assessment of tissue texture. International Journal of Molecular Medicine 14(5): 897-902, 2004