+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Proton-Resistant Quantum Dots: Stability in Gastrointestinal Fluids and Implications for Oral Delivery of Nanoparticle Agents

Proton-Resistant Quantum Dots: Stability in Gastrointestinal Fluids and Implications for Oral Delivery of Nanoparticle Agents

Nano Research 2(6): 500-508

Semiconductor quantum dots (QDs) have shown great promise as fluorescent probes for molecular, cellular and in-vivo imaging. However, the fluorescence of traditional polymer-encapsulated QDs is often quenched by proton-induced etching in acidic environments. This is a major problem for QD applications in the gastrointestinal tract because the gastric (stomach) environment is strongly acidic (pH 1-2). Here we report the use of proton-resistant surface coatings to stabilize QD fluorescence under acidic conditions. Using both hyperbranched polyethylenimine (PEI) and its polyethylene glycol derivative (PEG grafted PEI), we show that the fluorescence of core-shell CdSe/CdS/ZnS QDs is effectively protected from quenching in simulated gastric fluids. In comparison, amphiphilic lipid or polymer coatings provide no protection under similarly acidic conditions. The proton-resistant QDs are found to cause moderate membrane damage to cultured epithelial cells, but PEGylation (PEG grafting) can be used to reduce cellular toxicity and to improved nanoparticle stability.

(PDF emailed within 0-6 h: $19.90)

Accession: 055260682

Download citation: RISBibTeXText

PMID: 20379372

DOI: 10.1007/s12274-009-9046-3

Related references

The use of heat transfer fluids in the synthesis of high-quality CdSe quantum dots, core/shell quantum dots, and quantum rods. Nanotechnology 16(10): 2000-2011, 2005

Quantum dots as a platform for nanoparticle drug delivery vehicle design. Advanced Drug Delivery Reviews 65(5): 703-718, 2014

An Approach to the Design of a Particulate System for Oral Protein Delivery .II. Preparation and Stability Study of rhGH-Loaded Microspheres in Simulated Gastrointestinal Fluids. Iranian Journal of Pharmaceutical Research 10(2): 183-192, 2011

The Effects of Biological Fluids on Colloidal Stability and siRNA Delivery of a pH-Responsive Micellar Nanoparticle Delivery System. Acs Nano, 2017

Quantum dots: a powerful tool for understanding the intricacies of nanoparticle-mediated drug delivery. Expert Opinion on Drug Delivery 6(10): 1091-1112, 2009

An approach to the design of a particulate system for oral protein delivery. I. In vitro stability of various poly (alpha-hydroxy acids)-microspheres in simulated gastrointestinal fluids. Journal of Microencapsulation 25(8): 584-592, 2008

Core-shell nanocarriers with ZnO quantum dots-conjugated Au nanoparticle for tumor-targeted drug delivery. Carbohydrate Polymers 92(2): 1124-1132, 2013

Proton-sponge coated quantum dots for siRNA delivery and intracellular imaging. Journal of the American Chemical Society 130(28): 9006-9012, 2008

CdTe quantum dots induce activation of human platelets: implications for nanoparticle hemocompatibility. International Journal of Nanomedicine 10: 2723-2734, 2016

Development of biocompatible and proton-resistant quantum dots assembled on gelatin nanospheres. Langmuir 30(7): 1893-1899, 2014

Development of dual-emission ratiometric probe-based on fluorescent silica nanoparticle and CdTe quantum dots for determination of glucose in beverages and human body fluids. Food Chemistry 204: 444-452, 2016

Quantum Dots: Tracking the Down-Regulation of Folate Receptor-α in Cancer Cells through Target Specific Delivery of Quantum Dots Coupled with Antisense Oligonucleotide and Targeted Peptide (Small 24/2013). Small 9(24): 4182-4182, 2013

Loading Efficiency of Polymersomes with Contrast Agents and their Intracellular Delivery: Quantum Dots Versus Organic Dyes. Anticancer Research 38(2): 825-831, 2018

Energy Transfer Assays Using Quantum Dot-Gold Nanoparticle Complexes: Optimizing Oligonucleotide Assay Configuration Using Monovalently Conjugated Quantum Dots. Langmuir 31(29): 8194-8204, 2015

Expression of P-glycoprotein and CYP3A4 along the porcine oral-gastrointestinal tract: implications on oral mucosal drug delivery. Drug Development and Industrial Pharmacy 40(5): 599-603, 2015