Purification of EGCG monomer from the extracts of tea by high speed countercurrent chromatography

Zhang, Y.; Jiang, Y.-B.; Huang, S.-L.; Zheng, M.-Y.

Zhong Yao Cai 32(5): 784-787

2009


ISSN/ISBN: 1001-4454
PMID: 19771854
Accession: 055295350

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Abstract
To investigate the feasibility and techniques for purifying epigallocatechin-3-gallate (EGCG) by high speed countercurrent chromatography (HSCCC). A HSCCC unit was used for the purification of EGCG from the extracts of tea. The preferable solvent system was composed of Ether-Ethyl Acetate-H2O (4:10: 25, V/V), and the preferable technical conditions were as follows: the temperature was 35 degrees C, the rotation speed was 800 r/min and the mobile phase flow-rate was 2.5 mL/min. The purity of the obtained EGCG sample determined by high performance liquid chromatography (HPLC) is 95%.