+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)

Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)

International Immunopharmacology 17(3): 858-866

A water-soluble polysaccharide (JS-MP-1) was isolated and purified from the Korean mulberry fruits Oddi (Morus alba L.) by crushing the fresh fruits then performing ethanol precipitation and DEAE-cellulose ion exchange chromatography. The neutral monosaccharide composition of the purified JS-MP-1 was determined to be composed mainly of galactose (37.6%, in mole percent), arabinose (36.3%), and rhamnose (18.4%), while other major sugars such as glucose, xylose, mannose, and fucose were present as minor components. HPLC analysis revealed that JS-MP-1 contains both galacturonic acid (GalA) and glucuronic acid (GlcA) at approximately 4:1 in mole percent. Monosaccharide composition, Fourier-transform infrared (FTIR) analysis, biochemical analysis, and elemental analysis suggested that JS-MP-1 is an acidic heteropolysaccharide, most likely a rhamnoarabinogalacturonan type plant pectic polysaccharide, with an apparent molecular mass of 1600 kDa containing no, or if any, negligible level of sulfate esters and proteins. Enzyme-Linked Immunosorbent Assay and RT-PCR analysis demonstrated that JS-MP-1 significantly stimulates murine RAW264.7 macrophage cells to release chemokines (RANTES and MIP-1α) and proinflammatory cytokines (TNF-α and IL-6) and to induce the expression of iNOS and COX-2, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that the mulberry fruit-derived polysaccharide JS-MP-1 can act as a potent immunomodulator, and these observations may support the applicability of this polysaccharide as an immunotherapeutic adjuvant or the water extracts of the mulberry fruit as a beneficial health food.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 055296002

Download citation: RISBibTeXText

PMID: 24120956

DOI: 10.1016/j.intimp.2013.09.019

Related references

Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.). Carbohydrate Polymers 146: 187-196, 2016

Purification and characterization of lectins from mulberry seeds (Morus alba L.). Pakistan Journal of Biological Sciences 7(10): 1808-1813, 2004

In vitro and In vivo Antioxidant Activity of Flavonoid Extracted from Mulberry Fruit (Morus alba L.). Pharmacognosy Magazine 12(46): 128-133, 2016

Chemical characterization of cytotoxic indole acetic acid derivative from mulberry fruit (Morus alba L.) against human cervical cancer. Bioorganic Chemistry 76: 28-36, 2018

Mulberry latent virus isolated from mulberry morus alba. Annals of the Phytopathological Society of Japan 42(3): 304-309, 1976

Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves. Carbohydrate Polymers 128: 52-62, 2015

Novel anti-adherence activity of mulberry leaves: inhibition of Streptococcus mutans biofilm by 1-deoxynojirimycin isolated from Morus alba. Journal of Antimicrobial ChemoTherapy 62(4): 751-757, 2008

Sterols from mulberry morus alba fruit and seed oil. Soobshcheniya Akademii Nauk Gruzinskoi SSR 102(1): 169-172, 1981

Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits. Food Chemistry 103(4): 1380-1384, 2007

Purification and properties of urease from the leaf of mulberry, Morus alba. Phytochemistry 53(3): 325-330, 2000

A novel polysaccharide isolated from mulberry fruits (Murus alba L.) and its selenide derivative: structural characterization and biological activities. Food and Function 7(6): 2886-2897, 2016

Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chemistry 97(1): 25-31, 2006

Modification of sex expression and fruit set in mulberry morus alba linn by morphactin. Indian Journal of Sericulture 29(2): 297-299, 1990

The Mulberry (Morus alba L.) Fruit-A Review of Characteristic Components and Health Benefits. Journal of Agricultural and Food Chemistry 65(48): 10383-10394, 2017

Hypolipidemic and antioxidant effects of mulberry (Morus alba L.) fruit in hyperlipidaemia rats. Food and Chemical Toxicology 48(8-9): 2374-2379, 2010