+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7



Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7



Acta Crystallographica. Section F Structural Biology and Crystallization Communications 68(Pt 9): 1102-1105



Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDGΔ) and stoUDGΔ complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDGΔ and stoUDGΔ complexed with uracil, respectively.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055296150

Download citation: RISBibTeXText

PMID: 22949205

DOI: 10.1107/s1744309112030278


Related references

Purification, crystallization and preliminary X-ray analysis of the PCNA2-PCNA3 complex from Sulfolobus tokodaii strain 7. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 12): 1282-1284, 2009

Purification, crystallization and preliminary X-ray crystallographic analysis of ST1022, a putative member of the Lrp/AsnC family of transcriptional regulators isolated from Sulfolobus tokodaii strain 7. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 63(Pt 11): 964-966, 2007

Crystallization and preliminary X-ray analysis of the YjgF/YER057c/UK114-family protein ST0811 from Sulfolobus tokodaii strain 7. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 61(Pt 9): 828-830, 2005

High pressure refolding, purification, and crystallization of flavin reductase from Sulfolobus tokodaii strain 7. Protein Expression and Purification 84(2): 214-218, 2012

Crystal structure of family 4 uracil-DNA glycosylase from Sulfolobus tokodaii and a function of tyrosine 170 in DNA binding. Febs Letters 589(19 Pt B): 2675-2682, 2015

Crystallization and preliminary X-ray crystallographic study of a putative aspartyl-tRNA synthetase from the crenarchaeon Sulfolobus tokodaii strain 7. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 63(Pt 7): 608-612, 2007

Overexpression, purification, crystallization and preliminary X-ray analysis of uracil N-glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 62(Pt 12): 1231-1234, 2006

Crystallization and preliminary crystallographic analysis of type 1 RNase H from the hyperthermophilic archaeon Sulfolobus tokodaii 7. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 62(Pt 8): 781-784, 2006

Sulfolobus tokodaii sp. nov. (f. Sulfolobus sp. strain 7), a new member of the genus Sulfolobus isolated from Beppu Hot Springs, Japan. Extremophiles 6(1): 39-44, 2002

Crystallization and preliminary crystallographic studies of putative RNA 3'-terminal phosphate cyclase from the crenarchaeon Sulfolobus tokodaii. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 6): 565-570, 2009

Crystallization and preliminary X-ray diffraction analysis of three recombinant mutants of Vaccinia virus uracil DNA glycosylase. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 69(Pt 3): 295-301, 2013

Crystallization and preliminary crystallographic studies of putative threonyl-tRNA synthetases from Aeropyrum pernix and Sulfolobus tokodaii. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 64(Pt 10): 903-910, 2008

Crystallization and preliminary X-ray diffraction analysis of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua). Acta Crystallographica Section D Biological Crystallography 57(11): 1706-1708, 2001

Crystallization and preliminary X-ray analysis of the uracil-DNA glycosylase DNA repair enzyme from herpes simplex virus type 1. Journal of Molecular Biology 234(3): 910-912, 1993