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Quantifying the volume of single cells continuously using a microfluidic pressure-driven trap with media exchange

Quantifying the volume of single cells continuously using a microfluidic pressure-driven trap with media exchange

Biomicrofluidics 8(1): 011101

We demonstrate a microfluidic device capable of tracking the volume of individual cells by integrating an on-chip volume sensor with pressure-activated cell trapping capabilities. The device creates a dynamic trap by operating in feedback; a cell is periodically redirected back and forth through a microfluidic volume sensor (Coulter principle). Sieve valves are positioned on both ends of the sensing channel, creating a physical barrier which enables media to be quickly exchanged while keeping a cell firmly in place. The volume of individual Saccharomyces cerevisiae cells was tracked over entire growth cycles, and the ability to quickly exchange media was demonstrated.

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Accession: 055316711

Download citation: RISBibTeXText

PMID: 24753720

DOI: 10.1063/1.4867035

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