+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

RANKL is necessary and sufficient to initiate development of antigen-sampling M cells in the intestinal epithelium

RANKL is necessary and sufficient to initiate development of antigen-sampling M cells in the intestinal epithelium

Journal of Immunology 183(9): 5738-5747

Microfold cells (M cells) are specialized epithelial cells situated over Peyer's patches (PP) and other organized mucosal lymphoid tissues that transport commensal bacteria and other particulate Ags into intraepithelial pockets accessed by APCs. The TNF superfamily member receptor activator of NF-kappaB ligand (RANKL) is selectively expressed by subepithelial stromal cells in PP domes. We found that RANKL null mice have <2% of wild-type levels of PP M cells and markedly diminished uptake of 200 nm diameter fluorescent beads. Ab-mediated neutralization of RANKL in adult wild-type mice also eliminated most PP M cells. The M cell deficit in RANKL null mice was corrected by systemic administration of exogenous RANKL. Treatment with RANKL also induced the differentiation of villous M cells on all small intestinal villi with the capacity for avid uptake of Salmonella and Yersinia organisms and fluorescent beads. The RANK receptor for RANKL is expressed by epithelial cells throughout the small intestine. We conclude that availability of RANKL is the critical factor controlling the differentiation of M cells from RANK-expressing intestinal epithelial precursor cells.

(PDF emailed within 0-6 h: $19.90)

Accession: 055333185

Download citation: RISBibTeXText

PMID: 19828638

DOI: 10.4049/jimmunol.0901563

Related references

Antigen-sampling cells in the salmonid intestinal epithelium. Developmental and Comparative Immunology 34(7): 768-774, 2010

Thymic cortical epithelium is sufficient for the development of mature T cells in relB-deficient mice. Journal of Immunology 158(6): 2558-2566, 1997

The murine A33 antigen is expressed at two distinct sites during development, the ICM of the blastocyst and the intestinal epithelium. Mechanisms of Development 98(1-2): 111-114, November, 2000

Expression of A33 antigen, an intestinal epithelium specific protein for targeting therapy, in gastric cancer, intestinal metaplasia, and in normal gastric epithelium. Proceedings of the American Association for Cancer Research Annual Meeting 38(0): 31, 1997

Dendritic cells genetically modified to express CD40 ligand and pulsed with antigen can initiate antigen-specific humoral immunity independent of CD4+T cells. Nature Medicine 6(10): 1154-1159, October, 2000

MGL2 Dermal dendritic cells are sufficient to initiate contact hypersensitivity in vivo. Plos One 4(5): E5619-E5619, 2009

Small amounts of superantigen, when presented on dendritic cells, are sufficient to initiate T cell. Journal of Experimental Medicine 178(2): 633-642, 1993

Cell surface action of thrombin is sufficient to initiate division of chick cells. Cell 14(4): 811-824, 1978

The homeobox gene Pdx1 is sufficient for endoderm cells to initiate pancreas formation. Developmental Biology 222(1): 253, June 1, 2000

Intestinal villous M cells: an antigen entry site in the mucosal epithelium. Proceedings of the National Academy of Sciences of the United States of America 101(16): 6110-6115, 2004

Host B cells and splenic phagocytic cells are not required while host dendritic cells are sufficient to initiate GVHD across MiHAs. Blood 96(11 Part 2): 312b, November 16, 2000

Spermatogonial stem cells alone are not sufficient to re-initiate spermatogenesis in the rat testis following adjudin-induced infertility. International Journal of Andrology 35(1): 86-101, 2012

Impairing squamous differentiation by Klf4 deletion is sufficient to initiate tongue carcinoma development upon K-Ras activation in mice. Carcinogenesis 35(3): 662-669, 2014