+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

RAP-PCR fingerprinting reveals time-dependent expression of matrix-related molecules following stem-cell based TGFβ1-induced chondrocyte development

RAP-PCR fingerprinting reveals time-dependent expression of matrix-related molecules following stem-cell based TGFβ1-induced chondrocyte development

International Journal of Molecular Medicine 27(4): 519-523

Different approaches of engineering cartilage to treat defects in the articulating surfaces of the joints have been designed, which mainly use mesenchymal stem cells or autologous chondrocytes for in situ transplantation. However, these cells are poorly characterized with respect to viability, degree of differentiation and morphology or production of extracellular matrix. At present, one of the key approaches to generate chondrocytes is the stimulation of stem cells with transforming growth factor (TGF) β1. To characterize the molecular alterations occurring during the cellular transformation induced by TGF-β1 exposure, the differentiation process of bone marrow-derived stem cells into chondrocytes was investigated using an in vitro chondrogenesis model and the RNA arbitrarily primed PCR (RAP-PCR) fingerprinting technique. Distinct genes were found to be differentially regulated during chondrocyte development beginning on day 1: collagen type I, non-muscle myosin MYH9, followed by manganese superoxide dismutase and sodium-potassium ATPase on day 7. The results suggest that using RAP-PCR for differential display fingerprinting is a useful tool to investigate the differentiation process of bone marrow-derived stem cells following TGF-β1-stimulation.

(PDF emailed within 0-6 h: $19.90)

Accession: 055333258

Download citation: RISBibTeXText

PMID: 21286661

DOI: 10.3892/ijmm.2011.608

Related references

RAP-PCR fingerprinting reveals time-dependent expression of development-related genes following differentiation process of Bacillus thuringiensis. Canadian Journal of Microbiology 61(9): 683-690, 2016

Chondrogenesis of Embryonic Stem Cell-Derived Mesenchymal Stem Cells Induced by TGFβ1 and BMP7 Through Increased TGFβ Receptor Expression and Endogenous TGFβ1 Production. Journal of Cellular Biochemistry 118(1): 172-181, 2016

Reporter-based isolation of induced pluripotent stem cell- and embryonic stem cell-derived cardiac progenitors reveals limited gene expression variance. Circulation Research 107(3): 340-347, 2010

Incorporation of biomimetic matrix molecules in PEG hydrogels enhances matrix deposition and reduces load-induced loss of chondrocyte-secreted matrix. Journal of Biomedical Materials Research. Part A 97(3): 281-291, 2011

Matrix-induced autologous mesenchymal stem cell implantation versus matrix-induced autologous chondrocyte implantation in the treatment of chondral defects of the knee: a 2-year randomized study. Archives of Orthopaedic and Trauma Surgery 135(2): 251-263, 2015

Network theory inspired analysis of time-resolved expression data reveals key players guiding P. patens stem cell development. Plos One 8(4): E60494-E60494, 2013

Cancer stem cell differentiation: TGFβ1 and versican may trigger molecules for the organization of tumor spheroids. Oncology Reports 32(2): 641-649, 2015

Expression of extracellular matrix molecules and cell surface molecules in the olfactory nerve pathway during early development. Journal of Comparative Neurology 366(1): 1-14, 1996

Chondrocyte and mesenchymal stem cell-based therapies for cartilage repair in osteoarthritis and related orthopaedic conditions. Maturitas 78(3): 188-198, 2015

Expression of extracellular matrix molecules related to placental development. Placenta 14(4): A9, 1993

Deciphering chondrocyte behaviour in matrix-induced autologous chondrocyte implantation to undergo accurate cartilage repair with hyaline matrix. Pathologie-Biologie 60(3): 199-207, 2012

Stem cell and extracellular matrix-related molecules increase following melatonin treatment in the skin of postmenopausal rats. Cell Biology International 38(8): 924-932, 2015

Altered expression of matrix-related molecules in the development of chronic Thy1.1 nephritis. Nephron. Experimental Nephrology 104(4): E169-E182, 2006

Embryonic stem cell-based screen for small molecules: cluster analysis reveals four response patterns in developing neural cells. Current Medicinal Chemistry 20(5): 710-723, 2014

The influence of cell-matrix attachment and matrix development on the micromechanical environment of the chondrocyte in tissue-engineered cartilage. Tissue Engineering. Part A 20(23-24): 3112-3121, 2015