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RAPD analysis on the germplasm resources of Baphicacanthus cusia

RAPD analysis on the germplasm resources of Baphicacanthus cusia

Zhong Yao Cai 33(2): 183-186

To explore the genetic diversity of germplasm resources of Baphicacanthus cusia on molecular leve. The molecular biological technique-random amplified polymorphic DNA (RAPD) were used. 104 random decamer primers were screened for RAPD fragments of Baphicacanthus cusia. 422 DNA bands were amplified by 12 primers, 55.69% products were found to be polymorphic. Base on UPGMA cluster analysis, a DNA molecular dendrogram was established to discuss the genetic diversity of the germplasm resources of Baphicacanthus cusia. The genetic differences are related to morphological differences on a certain extant, but not to geographic regions. There actually existed much genetic diversity on molecular level among different natural populations of Baphicacanthus cusia.

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Accession: 055333283

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PMID: 20575408

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