EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

RAPD and barcode analyses of groupers of the genus Epinephelus



RAPD and barcode analyses of groupers of the genus Epinephelus



Genetics and Molecular Research 12(4): 5721-5732



The diversity of Epinephelus species was investigated throughout Thailand. Random amplified polymorphic DNA successfully produced 1300 bands that were phylogenetically informative and used to construct cladograms. Values of pairwise genetic similarity (S) within species ranged from 0.65 in E. erythrurus to 0.99 in E. malabaricus. The interspecific values of S ranged from 0.23 between E. malabaricus and E. bleekeri to 0.66 between E. coeruleopunctatus and E. erythrurus. The intraspecific nucleotide variation ranged from 0.037 to 0.159 in the mitochondrially encoded 16S RNA (MT-RNR2) region and from 0.003 to 0.157 for the mitochondrially encoded cytochrome c oxidase I (MT-CO1) region. All sequences were submitted individually to GenBank. The barcode sequences of Thai species of Epinephelus were aligned to the same species found in GenBank. For the MT-RNR2 gene region, intraspecific nucleotide variation ranged from 0.000 to 0.121, and interspecific nucleotide variation ranged from 0.003 to 0.146. For the MT-CO1 gene region, intraspecific nucleotide variation ranged from 0.000 to 0.140, and interspecific nucleotide variation ranged from 0.000 to 0.166. The MT-RNR2 data indicate that some species, including E. bleekeri from India and E. malabaricus from Thailand are not monophyletic. Additionally, the MT-CO1 data indicated that E. bleekeri, E. quoyanus and E. coeruleopunctatus are not monophyletic. The sequences of E. lanceolatus from each country are highly conserved, with genetic distances ranging from 0.000 to 0.003. Another important result from this study is that the barcode sequence from Thai E. erythrurus was previously not present in the GenBank.

(PDF emailed within 0-6 h: $19.90)

Accession: 055333288

Download citation: RISBibTeXText

PMID: 24301941

DOI: 10.4238/2013.November.18.21



Related references

Taxonomic relationship among seven species of groupers (genus Epinephelus; family Serranidae) as revealed by RAPD fingerprinting. Marine Biotechnology 6(3): 229-237, 2004

Western Atlantic serranid fishes (groupers) of the genus Epinephelus. Quarterly Journal of the Florida Academy of Sciences, 27: 17-30, 1964

Phylogenetic relationships among groupers (genus Epinephelus) based on mitochondrial cytochrome b DNA sequences. Journal of the Korean Fisheries Society. October; 375: 414-422, 2004

Successful hybridization of groupers Epinephelus coioides x Epinephelus lanceolatus using cryopreserved sperm. Aquaculture 320.1-2 (Oct 19): 106-112, 2011

Aspects of the reproduction of two groupers epinephelus guttatus and epinephelus striatus in the west indies. Bulletin of Marine Science 40(2): 220-230, 1987

Size weight ratio in mediterranean groupers epinephelus aeneus and epinephelus guaza. Cybium 3(11): 15-21, 1980

Ovarian and testicular intersexuality in two protogynous Mediterranean groupers, Epinephelus aeneus and Epinephelus guaza. Unknown, 1975

Experiments on grow out culture of groupers, Epinephelus malabaricus Schneider and Epinephelus tauvina Forskal. Indian Journal of Fisheries 52(4): 469-475, 2000

Experiments on grow out culture of groupers, Epinephelus malabaricus (Schneider) and Epinephelus tauvina (Forskal). Indian Journal of Fisheries 52(4): 469-475, 2005

Ovarian and testicular inter sexuality in 2 protogynous mediterranean groupers epinephelus aeneus and epinephelus guaza. Reinboth, R (Ed ) Intersexuality in The Animal Kingdom Symposium Mainz, West Germany, July 8-12, 1974 Xv+449p Illus Springer-Verlag: Heidelberg, West Germany; New York, N Y , U S A Isbn 3-540-07118-0; Isbn 0-387-07118-0 222-227, 1975

Technique of pellet implantation and preparation for induced sex reversal of the groupers Epinephelus salmonoides and Epinephelus fario. Bulletin of Taiwan Fisheries Research Institute, 47: 213-219, 1989

Betanodavirus-associated mortalities of adult wild groupers Epinephelus marginatus (Lowe) and Epinephelus costae (Steindachner) in Algeria. Journal of Fish Diseases 37(3): 273-278, 2014

Primary male development of two sequentially hermaphroditic groupers, Epinephelus akaara and Epinephelus awoara (Perciformes: Epinephelidae). Journal of Fish Biology 88(4): 1598-1613, 2016

Formation of diploid and triploid hybrid groupers (hybridization of Epinephelus coioides ♀ × Epinephelus lanceolatus ♂) and their 5S gene analysis. Bmc Genetics 17(1): 136-136, 2016