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RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day

RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day

Chemical Communications 51(1): 122-124

Cell-free transcription-translation systems offer an effective and versatile platform to explore the impact of genetic variations on protein function. We have developed a protocol for preparing linear, mutagenic DNA templates for direct use in the PURE system, enabling the fast and semi-quantitative evaluation of amino acid variations on catalytic activity and stereo-selectivity in native and engineered variants of Old Yellow Enzyme.

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Accession: 055333533

Download citation: RISBibTeXText

PMID: 25384037

DOI: 10.1039/c4cc08240k

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