+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells

Oncology Reports 31(4): 1738-1744

Frog ribonucleases have been demonstrated to have anticancer activities. However, whether RC-6 ribonuclease exerts anticancer activity on human embryonal carcinoma cells remains unclear. In the present study, RC-6 induced cytotoxicity in NT2 cells (a human embryonal carcinoma cell line) and our studies showed that RC-6 can exert anticancer effects and induce caspase-9 and -3 activities. Moreover, to date, there is no evidence that frog ribonuclease-induced cytotoxicity effects are related to cellular senescence. Therefore, our studies showed that RC-6 can increase p16 and p21 protein levels and induce cellular senescence in NT2 cells. Notably, similar to retinoic acid-differentiated NT2 cells, neuron-like morphology was found on some remaining live cells after RC-6 treatment. In conclusion, our study is the first to demonstrate that RC-6 can induce cytotoxic effects, caspase-9/-3 activities, cellular senescence and neuron-like morphology in NT2 cells.

(PDF emailed within 0-6 h: $19.90)

Accession: 055333710

Download citation: RISBibTeXText

PMID: 24535104

DOI: 10.3892/or.2014.3023

Related references

Sunitinib induces cellular senescence via p53/Dec1 activation in renal cell carcinoma cells. Cancer Science 104(8): 1052-1061, 2014

RNase MC2: a new Momordica charantia ribonuclease that induces apoptosis in breast cancer cells associated with activation of MAPKs and induction of caspase pathways. Apoptosis 17(4): 377-387, 2012

Progress and utility of investigation of embryonal carcinoma: differentiation from embryonal carcinoma to cells related neuron owing to retinoic acid. Seikagaku. Journal of Japanese Biochemical Society 74(7): 567-572, 2002

Retrovirus activation in embryonal carcinoma cells by cellular promoters. Genes & Development 3(12b): 2062-2071, 1989

miR-494-3p Induces Cellular Senescence and Enhances Radiosensitivity in Human Oral Squamous Carcinoma Cells. International Journal of Molecular Sciences 17(7): -, 2016

Capture of a cellular transcriptional unit by a retrovirus: mode of provirus activation in embryonal carcinoma cells. Journal of Virology 66(8): 4982-4991, 1992

Matrine induces apoptosis in gastric carcinoma cells via alteration of Fas/FasL and activation of caspase-3. Journal of Ethnopharmacology 123(1): 91-96, 2009

Chrysin induces cell apoptosis via activation of the p53/Bcl-2/caspase-9 pathway in hepatocellular carcinoma cells. Experimental and Therapeutic Medicine 12(1): 469-474, 2016

Ectopic AP4 expression induces cellular senescence via activation of p53 in long-term confluent retinal pigment epithelial cells. Experimental Cell Research 339(1): 135-146, 2016

Mollugin induces apoptosis in human Jurkat T cells through endoplasmic reticulum stress-mediated activation of JNK and caspase-12 and subsequent activation of mitochondria-dependent caspase cascade regulated by Bcl-xL. Toxicology and Applied Pharmacology 241(2): 210-220, 2009

Polyglucosamine CIG-4049 induces apoptosis via caspase-dependent manner without detectable activation of caspase-3 in human cervical carcinoma. FASEB Journal 15(4): A554, March 7, 2001

Thymoquinone induces cell death in human squamous carcinoma cells via caspase activation-dependent apoptosis and LC3-II activation-dependent autophagy. Plos One 9(7): E101579-E101579, 2015

6-Shogaol induces apoptosis in human colorectal carcinoma cells via ROS production, caspase activation, and GADD 153 expression. Molecular Nutrition & Food Research 52(5): 527-537, 2008

Supernatant of bacterial fermented soybean induces apoptosis of human hepatocellular carcinoma Hep 3B cells via activation of caspase 8 and mitochondria. Food and Chemical Toxicology 45(2): 303-314, 2006