EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

RCL2, a potential formalin substitute for tissue fixation in routine pathological specimens



RCL2, a potential formalin substitute for tissue fixation in routine pathological specimens



Histopathology 60(5): 804-815



To investigate RCL2 as a fixative for tissue fixation in routine histopathological examination and to assess tissue suitability for ancillary investigations.   Forty-nine samples from 36 fresh specimens were cut into three equal pieces and fixed in RCL2 diluted in 100% ethanol, RCL2 in 95% ethanol, or neutral buffered formalin as control. Suitability for microtomy, quality of histomorphology, histochemistry, immunohistochemistry, fluorescent and silver in-situ hybridization analysis and extracted genomic DNA were assessed. Microtomy was straightforward in most tissue blocks, but there was difficulty in cutting in approximately a quarter of samples, which required careful handling by an experienced technician. There were no significant differences in tissue morphology between RCL2- and formalin-fixed tissues (P=0.08). Generally, the quality of histochemical staining, immunohistochemistry and in-situ hybridization were comparable to that of formalin-fixed tissues. Inconsistent immunoreactivity was noted, however, with antibodies against pan-cytokeratin and progesterone receptor. Genomic DNA concentration was higher in RCL2-fixed tissues. Using RCL2 diluted in 95% ethanol did not affect fixation quality. RCL2 is a potential formalin substitute suitable as a fixative for use in routine histopathological examination; however, difficulty in microtomy and occasional discrepancies in immunohistochemical reactivity require further optimization of the methodology.

(PDF emailed within 0-6 h: $19.90)

Accession: 055333765

Download citation: RISBibTeXText

PMID: 22320393

DOI: 10.1111/j.1365-2559.2011.04127.x



Related references

An alternative fixative to formalin fixation for molecular applications: the RCL2(®)-CS100 approach. Methods in Molecular Biology 724: 297-307, 2011

Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids. Brain Pathology 20(6): 1010-1020, 2011

Tissue shrinkage after fixation with formalin injection of prostatectomy specimens. Virchows Archiv 449(3): 297-301, 2006

Making the Most of Pathological Specimens: Molecular Diagnosis in Formalin-Fixed, Paraffin Embedded Tissue. Current Drug Targets 13(12): 1475-1487, 2012

Making the most of pathological specimens: molecular diagnosis in formalin-fixed, paraffin embedded tissue. Current Drug Targets 13(12): 1475-1487, 2013

Immunohistochemistry of epithelial cell markers in normal and pathological colon mucosa. Comparison of results based on routine formalin- and cold ethanol-fixation methods. Histochemistry 67(1): 7-21, 1980

Imaging mass spectrometry of intact proteins from alcohol-preserved tissue specimens: bypassing formalin fixation. Journal of Proteome Research 7(8): 3543-3555, 2008

Experiences with reduced formalin fixation of specimens and formalin-free preservation of anatomic samples. Anatomia, Histologia, Embryologia 16(3): 250-253, 1987

Routine use of phenolized formalin in fixation of autopsy brain tissue to reduce risk of inadvertent transmission of Creutzfeldt-Jakob disease. New England Journal of Medicine 319(10): 654-654, 1988

Correcting the Shrinkage Effects of Formalin Fixation and Tissue Processing for Renal Tumors: toward Standardization of Pathological Reporting of Tumor Size. Journal of Cancer 6(8): 759-766, 2015

Systematic comparison of tissue fixation with alternative fixatives to conventional tissue fixation with buffered formalin in a xenograft-based model. Virchows Archiv 461(3): 259-269, 2012

Pathological evaluation of intervertebral disc tissue specimens after routine cervical and lumbar decompression. A cost-benefit analysis retrospective study. Surgical Neurology 56(4): 252-255, October, 2001

The duration of fixation in formalin influences the yield of HCV cDNA-PCR products from formalin-fixed, paraffin-embedded liver tissue. Journal of Hepatology 18(SUPPL 1): S100-S101, 1993

Effects of processing delay, formalin fixation, and immunohistochemistry on RNA Recovery From Formalin-fixed Paraffin-embedded Tissue Sections. Diagnostic Molecular Pathology 17(1): 51-58, 2008

Effects of processing delay, formalin fixation, and immunohistochemistry on RNA Recovery From Formalin-fixed Paraffin-embedded Tissue Sections. Diagnostic Molecular Pathology 17(1): 51-58, 2008