EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

RNase R is a highly unstable protein regulated by growth phase and stress


Rna 16(4): 667-672
RNase R is a highly unstable protein regulated by growth phase and stress
RNase R is an important exoribonuclease that participates in the degradation of structured RNAs in Escherichia coli. In earlier work, it was shown that RNase R levels increase dramatically under certain stress conditions, particularly during cold shock and stationary phase. However, the regulatory processes that lead to this elevation are not well understood. We show here that the increase in RNase R in stationary phase is unaffected by the global regulators, RpoS and (p)ppGpp, and that it occurs despite a major reduction in rnr message. Rather, we find that RNase R is a highly unstable protein in exponential phase, with a half-life of approximately 10 min, and that the protein is stabilized in stationary phase, leading to its relative increase. RNase R is also stabilized during cold shock and by growth in minimal medium, two other conditions that lead to its elevation. These data demonstrate that RNase R is subject to regulation by a novel, posttranslational mechanism that may have important implications for our complete understanding of RNA metabolism.

(PDF same-day service: $19.90)

Accession: 055338009

PMID: 20185542

DOI: 10.1261/rna.1981010



Related references

Identification of highly conserved regulatory domains and protein-binding sites in the promoters of the rat and human genes encoding the stress-inducible 78-kilodalton glucose-regulated protein. Molecular and Cellular Biology 8(10): 4579-4584, 1988

A stress-induced, developmentally regulated, highly polymorphic protein family in Pisum sativum L. Planta 91(1): 7-17, 1993

Occurrence of 2 ribosomal rnase depending on growth phase in yeast induction of rnase in glucose starved cells. Biochimica et Biophysica Acta 652(1): 129-138, 1981

OLE RNA, an RNA motif that is highly conserved in several extremophilic bacteria, is a substrate for and can be regulated by RNase P RNA. Proceedings of the National Academy of Sciences of the United States of America 104(19): 7815-7820, 2007

Expression of Ly-6, a marker for highly malignant murine tumor cells, is regulated by growth conditions and stress. International Journal Of Cancer. 77(2): 306-313, Y 17, 1998

The expression of LY-6, a marker for highly-malignant tumor cells, is regulated by cytokines and by growth and stress conditions. Cytokine 9(11): 897, 1997

Up-regulation of mRNA ventricular PRNP prion protein gene expression in air pollution highly exposed young urbanites: endoplasmic reticulum stress, glucose regulated protein 78, and nanosized particles. International Journal of Molecular Sciences 14(12): 23471-23491, 2014

Minor introns are embedded molecular switches regulated by highly unstable U6atac snRNA. Elife 2: E00780-E00780, 2013

Phase 1 Study of Safety, Tolerability, and Pharmacokinetics of PTC299, an Inhibitor of Stress-Regulated Protein Translation. Clinical Pharmacology in Drug Development 5(4): 296-305, 2016

Growth-regulated expression and G0-specific turnover of the mRNA that encodes URH49, a mammalian DExH/D box protein that is highly related to the mRNA export protein UAP56. Nucleic Acids Research 32(6): 1857-1865, 2004