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RT-PCR for tyrosinase expression as a molecular marker in malignant melanoma






Journal of B.U.On. 7(4): 325-330

RT-PCR for tyrosinase expression as a molecular marker in malignant melanoma

As the incidence of malignant melanoma steadily increases the need for markers in detection of early metastasis and guidance of therapy has become urgent. Presence of melanoma cells in patient peripheral blood, lymph nodes or bone marrow (BM) specimens could indicate tumour dissemination, and thus a high risk of metastasis. In 1991 coupled Reverse Transcription and Polymerase Chain Reaction (RT-PCR) was firstly used to detect tyrosinase mRNA in the peripheral blood of melanoma patients. Since then numerous studies have evaluated the significance of tyrosinase expression in bone marrow specimens, lymph nodes and blood from melanoma patients and there results indicate that this method might have important clinical applications in melanoma management.


Accession: 055338862

PMID: 17955575



Related references

Gradilone, A.; Cigna, E.; Aglianò, A.M.; Frati, L., 2010: Tyrosinase expression as a molecular marker for investigating the presence of circulating tumor cells in melanoma patients. In 1991, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) was introduced to assess the expression of Tyrosinase in the peripheral blood of melanoma patients, in order to identify the presence of Circulating Melanoma Cells. To date, hundred...

Eberle, J.; Garbe, C.; Wang, N.; Orfanos, C., E., 1995: Incomplete expression of the tyrosinase gene family (Tyrosinase, TRP-1, and TRP-2) in human malignant melanoma cells in vitro. Sequence analysis of two clones found repressed in melanoma cell lines in earlier studies showed 9F2 to be identical with the TRP-1 gene and 6F5 with TRP-2 containing a long untranslated 3' end. For further investigation of the expression of...

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Xu, X.; Zhang, P.J.; Elder, D.E., 2003: Tyrosinase expression in malignant melanoma, desmoplastic melanoma, and peripheral nerve tumors. Archives of Pathology & Laboratory Medicine 127(9): 1083-4; Author Reply 1084-5

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