Home
  >  
Section 56
  >  
Chapter 55,364

Rapid detection of anti-hepatitis a virus neutralizing antibodies in a microplate enzyme immunoassay

Azizi, A.; Sirskyj, D.; Weltzin, R.; Anderson, D.E.; Diaz-Mitoma, F.

Journal of Medical Microbiology 58(Part 11): 1433-1436

2009

ISSN/ISBN: 1473-5644
PMID: 19608692
DOI: 10.1099/jmm.0.012203-0
Accession: 055363193
The slow growth of hepatitis A virus (HAV) in cell culture is one of the primary pitfalls in the development of sensitive and rapid methods for the detection and quantification of HAV and associated neutralizing antibodies. Currently, in vitro assays frequently require 8 days or more to detect and quantify the presence of HAV neutralizing antibodies. This study describes a rapid immunoassay that allowed the detection of anti-HAV neutralizing antibodies in only 3 days. This microplate-based enzymic assay may be applicable in virological diagnostics, in evaluating the immunogenicity of HAV vaccines and in quantifying neutralizing antibodies during the course of HAV infection.

PDF emailed within 0-6 h: $19.90




Related References

Vlaspolder, F.; Harmsen, T.; van Veenendaal, D.; Kraaijeveld, C.A.; Snippe, H. 1988: Application of immunoassay of encephalomyocarditis virus in cell culture with enzyme-labeled virus-specific monoclonal antibodies for rapid detection of virus, neutralizing antibodies, and interferon Journal of Clinical Microbiology 26(12): 2593-2597
Harmsen, M.; Oosterlaken, T.A.; Tangerman, C.; Snippe, H.; Kraaijeveld, C.A. 1993: Detection by a solid phase independent enzyme immunoassay of monoclonal anti-idiotypic antibodies against monoclonal antibodies neutralizing Semliki Forest virus and encephalomyocarditis virus Journal of Virological Methods 43(2): 137-146
Van Tiel, F.H.; Harmsen, T.; Wagenaar, M.; Boere, W.A.; Kraaijeveld, C.A.; Snippe, H. 1986: Rapid determination of neutralizing antibodies to Semliki Forest virus in serum by enzyme immunoassay in cell culture with virus-specific monoclonal antibodies Journal of Clinical Microbiology 24(4): 665-668
D.M.dina M.; Ortiz, C.; Krenc, C.; Leete, J.; Hill, M.; Larue, S.; Schiff, E.; Perez, G. 1991: Improved detection of hepatitis c virus hcv antibodies in dialysis patients utilizing a second generation hcv enzyme immunoassay anti hcv eia Journal of the American Society of Nephrology 2(3): 320
Myrmel, H.; Navaratnam, V.; Asjø, B. 2005: Detection of antibodies to hepatitis C virus: false-negative results in an automated chemiluminescent microparticle immunoassay (ARCHITECT Anti-HCV) compared to a microparticle enzyme immunoassay (AxSYM HCV Version 3.0) Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology 34(3): 211-5; Discussion 216-8
Oosterlaken, T.A.; Harmsen, M.; Tangerman, C.; Schielen, P.; Kraaijeveld, C.A.; Snippe, H. 1988: A neutralization-inhibition enzyme immunoassay for anti-idiotypic antibodies that block monoclonal antibodies neutralizing Semliki Forest virus Journal of Immunological Methods 115(2): 255-261
Kudo, T.; Kido, A.; Nishiyama, Y.; Koganeya, H.; Okuda, T.; Nabeshima, M.; Iinuma, Y.; Ichiyama, S. 2004: Whole-blood counting immunoassay as a short-turnaround test for detection of hepatitis B surface antigen, anti-hepatitis C virus antibodies, and anti-Treponema pallidum antibodies Journal of Clinical Microbiology 42(9): 4250-4252
Gust, C.; Edwards, L.; Delgado, J.; Caldwell, S.; Murza, B.; Singh, P.; Kirchick, H. 1993: Rapid automated detection of IgM antibody to hepatitis A virus using radial partition enzyme immunoassay on the Stratus immunoassay system Abstracts of the General Meeting of the American Society for Microbiology 93: 447
Katz, D.; Freeman, E.; Davidson, M.; Abramson, M.; Fuchs, P. 1998: Detection of anti-rabies neutralizing antibodies in humans and cattle by a combined tissue culture and enzyme linked immunoassay Israel Journal of Veterinary Medicine 53(4): 132-142
Owolodun, O.A.; Giménez-Lirola, L.G.; Gerber, P.F.; Sanford, B.J.; Feagins, A.R.; Meng, X.-J.; Halbur, P.G.; Opriessnig, T. 2013: Development of a fluorescent microbead-based immunoassay for the detection of hepatitis e virus IgG antibodies in pigs and comparison to an enzyme-linked immunoassay Journal of Virological Methods 193(2): 278-283
Kraaijeveld, C.A.; Oosterlaken, T.A.; Vlaspolder, F.; van Dijk, P.W.; Harmsen, M.; Fernández, I.M.; Benaissa-Trouw, B.J.; Snippe, H. 1993: Detection of a shared idiotope on two encephalomyocarditis virus neutralizing monoclonal antibodies by neutralization inhibition enzyme immunoassay Journal of Virological Methods 44(2-3): 319-328
Pozzo, L.; Borla, M.; De Luca, M.; Calleri, M. 1999: Analytical performance of two improved microplate Enzyme Immunoassays used for the qualitative detection of total and IgM antibodies to hepatitis A virus Abstracts of the General Meeting of the American Society for Microbiology 99: 656-657
Döller, G.; Flehmig, B.; Schmitz, H. 1984: A comparison of enzyme-immunoassay and radioimmunoassay for detection of hepatitis a virus and antibodies against hepatitis a virus Journal of Biological Standardization 12(1): 47-59
Kramski, M.; Drozd, A.; Lichtfuss, G.F.; Dabrowski, P.W.; Ellerbrok, H. 2011: Rapid detection of anti-Vaccinia virus neutralizing antibodies Virology Journal 8: 139
Kruglov, I.V.; Znoĭko, O.O.; Klimova, E.A.; Finogenova, M.P.; Alekseenkova, T.I.; Ianina, M.V.; Voropaeva, A.V.; Voropaev, E.V.; Kalinin, A.L.; Doroshenko, N.V.; Shibnev, V.A.; Semiletov, I.A. 2003: Detection of type specific antibodies to hepatitis C virus NS4 protein in hepatitis C patients by enzyme immunoassay Voprosy Virusologii 48(3): 32-36