+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Recent advances in monolithic columns for protein and peptide separation by capillary liquid chromatography



Recent advances in monolithic columns for protein and peptide separation by capillary liquid chromatography



Analytical and Bioanalytical Chemistry 405(7): 2095-2106



Capillary liquid chromatography (cLC) has great potential for protein and peptide separation, with advantages of high efficiency, high resolution, low sample consumption, and high sensitivity when coupled with mass spectrometry. In recent years, monoliths have been widely used as the stationary phases for capillary columns, owing to easy preparation, high permeability, fast mass transfer, and low backpressure. This review summarizes recent advances (2007-2012) in monolithic columns for protein and peptide separation by cLC. After a brief introduction on the preparation of monolithic capillary columns, the emphasis of this review is focused on the recent application of such columns for protein and peptide separation by cLC. Furthermore, the challenges and potential hot points of monolithic capillary columns in the future are discussed.

(PDF emailed within 0-6 h: $19.90)

Accession: 055398188

Download citation: RISBibTeXText

PMID: 23208285

DOI: 10.1007/s00216-012-6570-x


Related references

Advances in capillary electrochromatography and micro-high performance liquid chromatography monolithic columns for separation science. Electrophoresis 24(6): 917-944, 2003

Comparing monolithic and microparticular capillary columns for the separation and analysis of peptide mixtures by liquid chromatography-mass spectrometry. Journal of Separation Science 28(14): 1666-1674, 2005

Porous structure of the monolithic sorbent and separation properties of monolithic capillary columns in gas and liquid chromatography. Russian Chemical Bulletin 59(8): 1660-1664, 2010

Affinity chromatography with monolithic capillary columns I. Polymethacrylate monoliths with immobilized mannan for the separation of mannose-binding proteins by capillary electrochromatography and nano-scale liquid chromatography. Journal of Chromatography. A 1044(1-2): 177-186, 2004

Recent advances in the preparation and application of monolithic capillary columns in separation science. Analytica Chimica Acta 931: 1-24, 2016

Affinity monolithic capillary columns for glycomics/proteomics: 1. Polymethacrylate monoliths with immobilized lectins for glycoprotein separation by affinity capillary electrochromatography and affinity nano-liquid chromatography in either a single column or columns coupled in series. Electrophoresis 27(5-6): 1020-1030, 2006

Surfactant-bound monolithic columns for separation of proteins in capillary high performance liquid chromatography. Journal of Chromatography. A 1217(4): 530-539, 2010

Separation of benzene and deuterated benzenes by reversed-phase and recycle liquid chromatography using monolithic capillary columns. Journal of Separation Science 27(15-16): 1339-1344, 2004

Hydroxymethyl methacrylate-based monolithic columns designed for separation of oligonucleotides in hydrophilic-interaction capillary liquid chromatography. Journal of Biochemical and Biophysical Methods 70(1): 23-29, 2007

Performance evaluation of long monolithic silica capillary columns in gradient liquid chromatography using peptide mixtures. Journal of Chromatography. A 1218(21): 3360-3366, 2011

Monolithic poly (SPE-co-BVPE) capillary columns as a novel hydrophilic interaction liquid chromatography stationary phase for the separation of polar analytes. Talanta 100: 344-348, 2013

Effects of the operation parameters on Hydrophilic Interaction Liquid Chromatography separation of phenolic acids on zwitterionic monolithic capillary columns. Journal of Chromatography. A 1217(51): 7981-7989, 2011

Affinity chromatography with monolithic capillary columns. II. Polymethacrylate monoliths with immobilized lectins for the separation of glycoconjugates by nano-liquid affinity chromatography. Journal of Chromatography. A 1079(1-2): 236-245, 2005

Protein-doped monolithic silica columns for capillary liquid chromatography prepared by the sol-gel method: applications to frontal affinity chromatography. Analytical Chemistry 76(10): 2780-2790, 2004

Cross-linker effects on the separation efficiency on (poly)methacrylate capillary monolithic columns. Part I. Reversed-phase liquid chromatography. Journal of Chromatography. A 1274: 97-106, 2013