Section 56
Chapter 55,429

Reduced uterine receptivity for mouse embryos developed from in-vitro matured oocytes

Lin, Y.-H.; Tsai, C.-Y.; Huang, L.-W.; Seow, K.-M.; Hwang, J.-L.; Tzeng, C.-R.

Journal of Assisted Reproduction and Genetics 31(12): 1713-1718


ISSN/ISBN: 1573-7330
PMID: 25292155
DOI: 10.1007/s10815-014-0354-5
Accession: 055428672

The outcomes of in-vitro maturation (IVM) are inferior compared to those of IVF. The purpose of the study was to compare the implantation rates of IVM- and in-vivo maturation (IVO)- derived embryos, and to evaluate their effects on uterine receptivity. The IVM- and IVO- oocytes were obtained from female mice, fertilized and transferred to separate oviducts of the same pseudo-pregnant mice. After 5 days, the implanted blastocysts were dissected out of the uterine horns, and the uterine horns were analyzed for the expression of mRNAs encoding leukemia inhibitory factor, heparin-binding epidermal growth factor, insulin-like growth factor binding protein-4, progesterone receptor, and Hoxa-10. The maturation rate of the IVM- oocytes was 81.2%. The fertilization rate of the IVM oocytes was lower than that of the IVO oocytes (50.5% vs. 78.0%, p = 0.038), as was their implantation rate (14.5% vs. 74.7%, p < 0.001). All 5 mRNAs examined were expressed at significantly lower levels in the uterine horns that received the IVM-derived embryos than in those that received the IVO-derived embryos. The IVM-derived embryos are less competent in inducing expression of implantation-related mRNAs in the uterine horn.

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