+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

RhoA is dispensable for axon guidance of sensory neurons in the mouse dorsal root ganglia

RhoA is dispensable for axon guidance of sensory neurons in the mouse dorsal root ganglia

Frontiers in Molecular Neuroscience 5: 67

RhoA, a member of the Rho family small GTPases, has been shown to play important roles in axon guidance. However, to date, the physiological function of RhoA in axon guidance events in vivo has not been determined genetically in animals. Here we show that RhoA mRNA is strongly expressed by sensory neurons in the developing mouse dorsal root ganglia (DRG). We have deleted RhoA in sensory neurons of the DRG using RhoA-floxed mice under the Wnt1-Cre driver in which Cre is strongly expressed in sensory neurons. Peripheral projections of sensory neurons appear normal and there are no detectable defects in the central projections of either cutaneous or proprioceptive sensory neurons in RhoA(f/f); Wnt1-Cre mice. Furthermore, a co-culture assay using DRG explants from RhoA(f/f); Wnt1-Cre embryos, and 293T cells expressing semaphorin3A (Sema3A) reveals that RhoA is not required for Sema3A-mediated axonal repulsion of sensory neurons. Expression of RhoC, a closely related family member, is increased in RhoA-deficient sensory neurons and may play a compensatory role in this context. Taken together, these genetic studies demonstrate that RhoA is dispensable for peripheral and central projections of sensory neurons in the DRG.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 055570576

Download citation: RISBibTeXText

PMID: 22661927

DOI: 10.3389/fnmol.2012.00067

Related references

Downregulation of adult and neonatal Nav1.5 in the dorsal root ganglia and axon of peripheral sensory neurons of rats with spared nerve injury. International Journal of Molecular Medicine 41(4): 2225-2232, 2018

Neuropilin-mediated neural crest cell guidance is essential to organise sensory neurons into segmented dorsal root ganglia. Development 136(11): 1785-1789, 2009

Caspase-mediated apoptosis in sensory neurons of cultured dorsal root Ganglia in adult mouse. Cell Journal 15(3): 212-217, 2013

Sdf - 1/cxcr4 signaling regulates the migration and development of nociceptive sensory neurons in mouse dorsal root ganglia. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 140 5, 2003

Adeno-associated virus and lentivirus vectors mediate efficient and sustained transduction of cultured mouse and human dorsal root ganglia sensory neurons. Human Gene Therapy 12(1): 77-86, 2001

Inhibition of c-Jun phosphorylation reduces axonal outgrowth of adult rat nodose ganglia and dorsal root ganglia sensory neurons. Molecular and Cellular Neurosciences 27(3): 267-279, 2004

Localization and comparative analysis of acid-sensing ion channel (ASIC1, 2, and 3) mRNA expression in mouse colonic sensory neurons within thoracolumbar dorsal root ganglia. Journal of Comparative Neurology 500(5): 863-875, 2006

Dorsal root ganglia sensory neurons. Methods in Molecular Biology 229: 155-167, 2003

Distribution of sensory neurons of ventral and dorsal cervical cutaneous nerves in dorsal root ganglia of adult rat--a double-label study using DiO and DiI. Okajimas Folia Anatomica Japonica 79(5): 129-133, 2003

Histone acetylation inhibitors promote axon growth in adult dorsal root ganglia neurons. Journal of Neuroscience Research 93(8): 1215-1228, 2016

The role of neurotrophins in the maintenance of the spinal cord motor neurons and the dorsal root ganglia proprioceptive sensory neurons. International Journal of Developmental Neuroscience 23(7): 613-620, 2005

Postmitotic primary sensory neurons from adult mouse dorsal root ganglia are immortalized by a temperature-sensitive A58 SV40 large T antigen and undergo extensive apoptosis when grown under non-permissive conditions. Molecular Biology of the Cell 8(SUPPL ): 34A, 1997

Localization of renal sensory neurons in the dorsal root ganglia of the rat. Anatomical Record 202(3): 48A, 1982

Phenotypes of sensory neurons in the chick dorsal root ganglia. Journal of Neurochemistry 44(SUPPL): S43, 1985

Projections of sensory neurons in transplanted dorsal root ganglia. Society for Neuroscience Abstracts 10(2): 1034, 1984