+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Robust one-day in situ hybridization protocol for detection of micro RNAs in paraffin samples using LNA probes

Robust one-day in situ hybridization protocol for detection of micro RNAs in paraffin samples using LNA probes

Methods 52(4): 375-381

ISSN/ISBN: 1046-2023

PMID: 20621190

DOI: 10.1016/j.ymeth.2010.07.002

MicroRNAs (miRNAs) constitute a group of small non-coding RNA molecules generally 18-22 base-pairs in length. miRNAs are considered to be negative regulators of gene expression at the level of post-transcription and are important in normal physiological development, tissue homeostasis and disease. The cellular origin of individual microRNAs is of utmost importance for understanding their roles in molecular and biological processes, in multi-cellular and complex structured tissues. For the localization of miRNAs in clinical and experimental formalin-fixed and paraffin-embedded samples we have developed a simple and robust one-day in situ hybridization protocol based on the use of double digoxigenin (DIG)-labeled LNA-DNA chimeric probes. We show that the protocol enables analyses of specificity, and demonstrate the detection of miR-1, miR-21, miR-124, miR-126, miR-145, and miR-205 in human and murine paraffin material. The well established localization of these microRNAs makes them ideal for use as reference microRNAs when optimizing the microRNA in situ hybridization assay for a particular tissue and miRNA.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 055594504

Download citation: RISBibTeXText

Related references

Comparison of peroxidase-labeled DNA probes with radioactive RNA probes for detection of human papillomaviruses by in situ hybridization in paraffin sections. Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 4(1): 81-85, 1991

Detection of micro RNAs in cultured cells and paraffin-embedded tissue specimens by in situ hybridization. Methods in Molecular Biology 676: 73-83, 2011

In situ hybridization for the detection of cytomegalovirus (CMV) infection. Application of biotinylated CMV-DNA probes on paraffin-embedded specimens. Virchows Archiv. A Pathological Anatomy and Histopathology 409(6): 777-790, 1986

Effects of different fixatives on detection of nucleic acids from paraffin-embedded tissues by in situ hybridization using oligonucleotide probes. Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society 39(9): 1237-1242, 1991

Optimization of in situ hybridization assay using non-radioactive DNA probes for the detection of canine herpesvirus (CHV) in paraffin-embedded sections. Journal of Veterinary Science 5(1): 71-73, 2004

Effects of different fixatives on detection of nucleic acids from paraffin-embeded tissues by in situ hybridization using oligonucleotide probes. The Journal of Histochemistry and Cytochemistry 39(9): 1237-1242, 1991

Detection of t (11; 18) and rearrangement of bcl-10 in paraffin-embedded samples using interphase fluorescence in-situ hybridization. Zhonghua Bing Li Xue Za Zhi 36(7): 494-495, 2007

Influence of fixation on human papillomavirus DNA detection in frozen and embedded paraffin lesions by in situ hybridization with biotinylated probes. Pathology Research and Practice 188(8): 1018-1027, 1992

In situ Hybridization for the Detection of Feline Interleukin 1.Alpha. mRna on the Paraffin-Embeddeb Section using Biotin-Labeled Probes. The Journal of Veterinary Medical Science 53(3): 451-456, 1991

In situ hybridization for the detection of feline interleukin 1 alpha mRNA on the paraffin-embedded section using biotin-labeled probes. Journal of Veterinary Medical Science 53(3): 451-456, 1991

An improved in situ DNA hybridization protocol for detection of human papillomavirus (HPV) DNA sequences in paraffin-embedded biopsies. Journal of Virological Methods 14(3-4): 293-304, 1986

Detection by the fluorescence in situ hybridization technique of MYC translocations in paraffin-embedded lymphoma biopsy samples. British Journal of Haematology 121(1): 49-56, 2003

In situ hybridization detection of varicella zoster virus in paraffin-embedded skin biopsy samples. Clinical and Diagnostic Virology 7(2): 69-76, 1996

Evaluation of the validity of human papillomavirus detection in paraffin-embedded cervical samples by the DNA hybridization in situ. Acta Universitatis Palackianae Olomucensis Facultatis Medicae 136: 19-21, 1993

Detection of N-myc gene amplification on isolated nuclei from paraffin-embedded neuroblastoma samples by fluorescence in situ hybridization. Modern Pathology 8(1): 4P, 1995