+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Role of calcitriol and cortisol on human adipocyte proliferation and oxidative and inflammatory stress: a microarray study

Role of calcitriol and cortisol on human adipocyte proliferation and oxidative and inflammatory stress: a microarray study

Journal of Nutrigenetics and Nutrigenomics 1(1-2): 30-48

Dietary calcium inhibits adiposity, and a key underlying mechanism is suppression of calcitriol, which modulates Ca(2+) signaling and mitochondrial uncoupling in adipocytes. We demonstrated that calcitriol directly regulates adipocyte 11beta-HSD-1 expression and cortisol production in human adipocytes in vitro and dietary calcium inhibits visceral adipose tissue 11beta-HSD-1 expression in mice, indicating an interaction of calcitriol and cortisol in obesity. Consequently, we have evaluated the gene expression profile of human subcutaneous adipocytes treated with calcitriol and/or cortisone. Data analysis demonstrated significant calcitriol modulation of gene expression toward inhibition of the adipocyte apoptosis (e.g., VEGF and STC-2) and promotion of adipocyte proliferation (e.g., IGF-1 and IGF-1R). Calcitriol also up-regulated oxidative stress and inflammatory genes such as NOX-4 and TLR-3. The calcitriol/cortisone combination resulted in significant additional up-regulation of 11beta-HSD-1 and down-regulation of adiponectin expression, while cortisone exerted little independent effect in the absence of calcitriol. Overall, calcitriol stimulated a pattern of adipocyte gene expression which favored adipocyte proliferation, oxidative and inflammatory stress and visceral adiposity, and these effects were amplified in the presence of cortisone; however, this conclusion must be tempered by the adipocyte source (subcutaneous) and requires confirmation in visceral adipocytes.

(PDF emailed within 0-6 h: $19.90)

Accession: 055601912

Download citation: RISBibTeXText

PMID: 19918113

DOI: 10.1159/000109873

Related references

Elucidating the Range of Oxidative Stress Pathways in Human Lens Epithelial Cells A cDNA Microarray Study. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 851, 2002

Oxidative stress and adipocyte biology: focus on the role of AGEs. Oxidative Medicine and Cellular Longevity 2015: 534873-534873, 2015

The role of oxidative stress in pro-inflammatory activation of human endothelial cells on Ti6Al4V alloy. Biomaterials 34(33): 8075-8085, 2014

Putative role for oxidative stress in adipocyte and skeletal muscle insulin resistance. Diabetologia 41(SUPPL 1): A34, 1998

Oxidative stress mediates through apoptosis the anticancer effect of phospho-nonsteroidal anti-inflammatory drugs: implications for the role of oxidative stress in the action of anticancer agents. Journal of Pharmacology and Experimental Therapeutics 338(3): 775-783, 2011

Expression signatures, barriers and beyond: the role of oxidative stress in murine colitis and human inflammatory bowel disease revisited. European Journal of Gastroenterology & Hepatology 20(6): 496-499, 2008

The role of oxidative stress in hormesis induced by sodium arsenite in human embryo lung fibroblast (HELF) cellular proliferation model. Journal of Toxicology and Environmental Health. Part A 70(11): 976-983, 2007

Herbal Formulation C168 Attenuates Proliferation and Induces Apoptosis in HCT 116 Human Colorectal Carcinoma Cells: Role of Oxidative Stress and DNA Damage. Evidence-Based Complementary and Alternative Medicine 2016: 2091085-2091085, 2016

The role of microRNA-26b in human adipocyte differentiation and proliferation. Gene 533(2): 481-487, 2014

Molecular mechanisms of human lipodystrophies: from adipocyte lipid droplet to oxidative stress and lipotoxicity. International Journal of Biochemistry & Cell Biology 43(6): 862-876, 2011

Proliferation in hyperplastic human and normal rat parathyroid glands: role of phosphate, calcitriol, and gender. Kidney International 64(6): 2311-2317, 2003

Protective role of myricetin on markers of oxidative stress in human erythrocytes subjected to oxidative stress. Natural Product Communications 4(2): 221-226, 2009

Comparative microarray analysis of the patch and plaque stages of Mycosis Fungoides Implicating a role for oxidative stress?. Journal of Investigative Dermatology 121(1): 0201, July, 2003

The role of the stress-related anti-inflammatory hormones ACTH and cortisol in atherosclerosis. Current Vascular Pharmacology 8(4): 517-525, 2010

Microarray analyses support a role for Nurr1 in resistance to oxidative stress and neuronal differentiation in neural stem cells. Stem Cells 25(2): 511-519, 2006