+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Role of nitric oxide on quality of freshly ejaculated bull spermatozoa during heparin-induced in vitro capacitation



Role of nitric oxide on quality of freshly ejaculated bull spermatozoa during heparin-induced in vitro capacitation



Animal Reproduction Science 116(1-2): 38-49



The objective of this study was to assess the effects of nitric oxide (NO) on heparin-induced capacitation in vitro of fresh bull sperm, through the addition of Nomega-nitro-l-arginine methyl ester (L-NAME, a NO-synthesis inhibitor) and l-arginine (L-Arg, a NO-synthesis precursor) to the capacitation medium. In Experiment 1, different concentrations of L-NAME (0.1, 1, 10mM) and of L-Arg (10mM) were added to the capacitation medium. Sperm motility and vigor were subjectively appraised using direct light microscopy; sperm membrane integrity was examined using a 2% Trypan blue solution while the concentration of nitrate/nitrite (NO(3)(-)/NO(2)(-)) was determined by using the Griess method over a 5h capacitation period. The addition of 10mM L-NAME has inhibited NO synthesis, sperm progressive motility, sperm vigor and sperm membrane integrity (P<0.05) as compared to control. The addition of 10mM L-Arg to the capacitation medium increased all variables evaluated in comparison to the control (P<0.05). In Experiment 2, mitochondrial activity was assessed through the MTT test (3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and sperm capacitation was assessed through the test of penetration in homologous oocytes after addition of the 10mM L-NAME, and of the 10mM L-Arg. The addition of 10mM L-NAME caused mitochondrial activity (40%) and the percentage of oocytes penetrated (77%) to decrease in relation to the control (P<0.05). After addition of 0.6mM L-Arg+10mM L-NAME, partial reversal of mitochondrial activity did occur (only 20%). The addition of 10mM L-Arg increased the percentage of oocytes penetrated as compared to control (21%) (P<0.05). These results indicate that: (1) NO is involved in control of progressive sperm motility, vigor, membrane integrity, and mitochondrial activity along the period of heparin-induced capacitation of fresh bovine sperm via NOS/NO; (2) adequate L-Arg/NO concentrations into the capacitation medium can potentiate heparin action or act independently for increasing the number or the quality of capacitated sperm.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055608946

Download citation: RISBibTeXText

PMID: 19188034

DOI: 10.1016/j.anireprosci.2008.12.020


Related references

Role of nitric oxide on quality of freshly-ejaculated bull spermatazoa during heparin-induced in vitro capacitation. 2009

Nitric oxide-induced capacitation of cryopreserved bull spermatozoa and assessment of participating regulatory pathways. Animal Reproduction Science 85(3-4): 231-242, 2005

Nitric oxide and superoxide anion production during heparin-induced capacitation in cryopreserved bovine spermatozoa. Reproduction in Domestic Animals 46(1): 74-81, 2011

Detection of in vitro capacitation of bull spermatozoa following heparin treatment. Archiv fur Experimentelle Veterinarmedizin 43(4): 623-635, 1989

Individual differences in capacitation of bull spermatozoa by heparin in vitro: relationship to fertility. Theriogenology 34(3): 619-628, 1990

Heparin and dermatan sulphate induced capacitation of frozen-thawed bull spermatozoa measured by merocyanine-540. Zygote 15(3): 225-232, 2007

Heparin binding proteins from seminal plasma potentiate heparin induced capacitation of bovine spermatozoa in vitro. Biology of Reproduction 40(Suppl. 1): 148, 1989

In vitro capacitation of ejaculated rabbit spermatozoa. Differentiation: 125-132, 1979

Effects of medium containing heparin and theophylline on capacitation and metabolic enzyme activities of ejaculated spermatozoa from dogs with asthenozoospermia. Animal Reproduction Science 54(4): 251-259, 1999

Inhibition of in vitro capacitation of hamster spermatozoa by nitric oxide synthase inhibitors. Cellular and Molecular Biology 49(3): 421-428, 2003

Protein-leakage during heparin - induced in-vitro capacitation of bull sperm. Archiv fuer Tierzucht 49(5): 426-433, 2006

Factors influencing in vitro capacitation of ejaculated rabbit spermatozoa. Theriogenology 21(1): 245, 1984

Effects of in vitro capacitation on the chromatin stability of ejaculated spermatozoa. Contraception Fertilite Sexualite 16(7-8): 559-561, 1988

Cell calcium of ejaculated rabbit spermatozoa before and following in vitro capacitation. Biochemical and Biophysical Research Communications 134(2): 664-670, 1986

In vitro capacitation of ejaculated spermatozoa and in vitro fertilization in dairy goats. Theriogenology 35(1): 219, 1991