+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Role of the type III secretion system in a hypervirulent lineage of Bordetella bronchiseptica



Role of the type III secretion system in a hypervirulent lineage of Bordetella bronchiseptica



Infection and Immunity 77(9): 3969-3977



Despite the fact that closely related bacteria can cause different levels of disease, the genetic changes that cause some isolates to be more pathogenic than others are generally not well understood. We use a combination of approaches to determine which factors contribute to the increased virulence of a Bordetella bronchiseptica lineage. A strain isolated from a host with B. bronchiseptica-induced disease, strain 1289, was 60-fold more virulent in mice than one isolated from an asymptomatically infected host, strain RB50. Transcriptome analysis and quantitative reverse transcription-PCR showed that the type III secretion system (TTSS) genes were more highly expressed by strain 1289 than strain RB50. Compared to strain RB50, strain 1289 exhibited greater TTSS-mediated cytotoxicity of a mammalian cell line. Additionally, we show that the increase in virulence of strain 1289 compared to that of RB50 was partially attributable to the TTSS. Using multilocus sequence typing, we identified another strain from the same lineage as strain 1289. Similar to strain 1289, we implicate the TTSS in the increased virulence of this strain. Together, our data suggest that the TTSS is involved in the increased virulence of a B. bronchiseptica lineage which appears to be disproportionately associated with disease. These data are consistent with the view that B. bronchiseptica lineages can have different levels of virulence, which may contribute to this species' ability to cause different severities of respiratory disease.

(PDF emailed within 0-6 h: $19.90)

Accession: 055614670

Download citation: RISBibTeXText

PMID: 19596779

DOI: 10.1128/IAI.01362-08


Related references

In-vitro and in-vivo analysis of the production of the Bordetella type three secretion system effector A in Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Microbes and Infection 15(5): 399-408, 2013

Identification of a type III secretion system in bordetella bronchiseptica. Abstracts of the General Meeting of the American Society for Microbiology 98: 65, 1998

Iron starvation regulates the type III secretion system in Bordetella bronchiseptica. Microbiology and Immunology 56(6): 356-362, 2012

The BvgAS virulence control system regulates type III secretion in Bordetella bronchiseptica. Molecular Microbiology 28(5): 945-959, 1998

The Bordetella bronchiseptica Type III secretion system induces necrotic cell death. Abstracts of the General Meeting of the American Society for Microbiology 102: 92, 2002

Detection of type III secretion system genes in animal isolates of Bordetella bronchiseptica. Veterinary Microbiology 72(3/4): 329-337, 2000

A Type VI secretion system encoding locus is required for Bordetella bronchiseptica immunomodulation and persistence in vivo. Plos One 7(10): E45892-E45892, 2013

The Bordetella bronchiseptica type III secretion system is required for persistence and disease severity but not transmission in swine. Infection and Immunity 82(3): 1092-1103, 2014

Bordetella bronchiseptica Bcr4 antagonizes the negative regulatory function of BspR via its role in type III secretion. Microbiology and Immunology 2018: -, 2018

Differential regulation of type III secretion and virulence genes in Bordetella pertussis and Bordetella bronchiseptica by a secreted anti-σ factor. Proceedings of the National Academy of Sciences of the United States of America 113(9): 2341-2348, 2016

Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection. Plos One 10(10): E0140743-E0140743, 2016

Phenotypic and genomic analysis of hypervirulent human-associated Bordetella bronchiseptica. Bmc Microbiology 12: 167-167, 2013

The Bordetella bronchiseptica type III secretion system inhibits gamma interferon production that is required for efficient antibody-mediated bacterial clearance. Infection and Immunity 74(2): 1043-1049, 2006

Downregulation of mitogen-activated protein kinases by the Bordetella bronchiseptica Type III secretion system leads to attenuated nonclassical macrophage activation. Infection and Immunity 73(1): 308-316, 2004

An autotransporter is required for type III secretion in Bordetella bronchiseptica. Abstracts of the General Meeting of the American Society for Microbiology 103: B-269, 2003