EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Role of β-conglycinin and glycinin subunits in the pH-shifting-induced structural and physicochemical changes of soy protein isolate


Role of β-conglycinin and glycinin subunits in the pH-shifting-induced structural and physicochemical changes of soy protein isolate



Journal of Food Science 76(2): C293-C302



ISSN/ISBN: 0022-1147

PMID: 21535749

DOI: 10.1111/j.1750-3841.2010.02035.x

Soy β-conglycinin (7S) and glycinin (11S) were incubated up to 4 h in acidic (pH 1.5 to 3.5) or alkaline (pH 10 to 12) solutions to induce protein structural unfolding followed by refolding 1 h at pH 7.0, a process known as pH-shifting. The pH-shifting markedly increased (P < 0.05) emulsifying activity of 11S and to a lesser extent 7S; the former also produced more uniform oil droplets. The emulsifying activity improvements were accompanied by a significant rise in protein surface hydrophobicity, slight loss of the secondary structure (circular dichroism), and substantial dissociation of disulfide-linked basic and acidic 11S subunits. The findings suggested that 11S globulins of soy protein isolate (SPI) were more responsive to pH-shifting treatments than were 7S globulins, and the resulting emulsifying activity enhancements of 11S, in parallel with that of SPI, were indicative of its determinant role in the overall emulsifying properties of pH-shifting-treated SPI. Extreme alkaline (pH 12) and acidic (pH 1.5) medium treatments can significantly modify the structure and enhance the emulsifying properties of both β-conglycinin and glycinin components of SPI. The functionality improvement by the pH processes is more remarkable for the glycinin protein fraction. Therefore, SPI enriched with glycinin seems to be particularly suitable for extreme acidic or alkaline processes to produce surface-active functional ingredients for food applications.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055615993

Download citation: RISBibTeXText

Related references

Formation and properties of glycinin-rich and beta -conglycinin-rich soy protein isolate gels induced by microbial transglutaminase. Food Research International 39(1): 87-97, 2006

Identification of glycinin and beta-conglycinin subunits that contribute to the increased protein content of high-protein soybean lines. Journal of Agricultural and Food Chemistry 55(5): 1839-1845, 2007

Opposite Contributions of Glycinin- and β-Conglycinin-Derived Peptides to the Aggregation Behavior of Soy Protein Isolate Hydrolysates. Food Biophysics 1(4): 178-188, 2006

Selective Proteolysis of the Glycinin and b-Conglycinin Fractions in a Soy Protein Isolate by Pepsin and Papain with Controlled pH and Temperature. Journal of Food Science 69(5): 63-C367, 2004

Methodology for identification of pore forming antimicrobial peptides from soy protein subunits β-conglycinin and glycinin. Peptides 85: 27-40, 2016

Improvement of the nutritional value of soybean seed with alteration in protein subunits of glycinin and beta -conglycinin. Turkish Journal Of Biology: 2, 91-97, 2008

Selective proteolysis of the glycinin and beta-conglycinin fractions in a soy protein isolate by pepsin and papain with controlled pH and temperature. Journal of food science- y 69(5): C363-C367, 2004

Inhibition or improvement for acidic subunits fibril aggregation formation from beta-conglycinin, glycinin and basic subunits. Journal of Cereal Science 70: 263-269, 2016

Urea-elicited changes in relative electrophoretic mobility of certain glycinin and b-conglycinin subunits. Plant Physiology 76: 0-2, 1984

Urea-elicited changes in relative electrophoretic mobility of certain glycinin and β-conglycinin subunits. Plant Physiology (Bethesda) 76(3): 840-842, 1984

Urea-Elicited Changes in Relative Electrophoretic Mobility of Certain Glycinin and beta-Conglycinin Subunits. Plant Physiology 76(3): 840-842, 1984

The α' subunit of β-conglycinin and the A1-5 subunits of glycinin are not essential for many hypolipidemic actions of dietary soy proteins in rats. European Journal of Nutrition 53(5): 1195-1207, 2014

The α' subunit of β-conglycinin and various glycinin subunits of soy are not required to modulate hepatic lipid metabolism in rats. European Journal of Nutrition 57(3): 1157-1168, 2018

Genetic analysis of subunits of two major storage proteins (β-conglycinin and glycinin) in soybean seeds. Ikushugaku Zasshi 36(4): 390-400, 1986

Genetic analysis of subunits of two major storage proteins beta conglycinin and glycinin in soybean seeds. Japanese Journal of Breeding 36(4): 390-400, 1986