+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Screening and identification of radical scavengers from Neo-Taraxacum siphonanthum by online rapid screening method and nuclear magnetic resonance experiments

Screening and identification of radical scavengers from Neo-Taraxacum siphonanthum by online rapid screening method and nuclear magnetic resonance experiments

Journal of Immunoassay and Immunochemistry 31(3): 233-249

An online rapid screening method, the high-performance liquid chromatography (HPLC)-diode array detector (DAD)-radical scavenging detection (RSD)-electrospray ionization (ESI)-mass spectroscopy (MS)/MS system, was developed for the screening and identification of radical scavengers from Neo-Taraxacum siphonanthum, a new species found in China in 1989. For further characterization, the target compounds were isolated by silica column chromatography, preparative high-performance liquid chromatography (HPLC), HSCCC, and Sephadex LH-20 column chromatography and elucidated on the basis of ultraviolet (UV), ESI-MS/MS, and nuclear magnetic resonance (NMR) spectroscopy, as well as the chemical analysis. Eighteen antioxidative polyphenols (5 caffeic acid derivatives and 13 flavonoid derivatives) were characterized from Neo-T. siphonanthum. The distribution of all compounds was discussed in a chemosystematic context, which suggested that the genera Neo-Taraxacum and Taraxacum might relate chemosystematically.

(PDF emailed within 0-6 h: $19.90)

Accession: 055670213

Download citation: RISBibTeXText

PMID: 20623409

DOI: 10.1080/10739149.2010.488616

Related references

Identification of antioxidants from Taraxacum mongolicum by high-performance liquid chromatography-diode array detection-radical-scavenging detection-electrospray ionization mass spectrometry and nuclear magnetic resonance experiments. Journal of Chromatography. a 1209(1-2): 145-152, 2008

Efficient method for screening and identification of radical scavengers in the leaves of Olea europaea L. Biomedical Chromatography 25(3): 373-380, 2011

Acetogenic isoquinoline alkaloids. CXXI. Use of on-line high-performance liquid chromatography-nuclear magnetic resonance spectrometry coupling in phytochemical screening studies: Rapid identification of metabolites in Dioncophyllum thollonii. Journal of Chromatography A 837(1-2): 267-272, April 2, 1999

High-Throughput Screening by Nuclear Magnetic Resonance (HTS by NMR) for the Identification of PPIs Antagonists. Current Topics in Medicinal Chemistry 15(20): 2032-2042, 2016

Low field nuclear magnetic resonance for rapid quantitation of microalgae lipid and its application in high throughput screening. Sheng Wu Gong Cheng Xue Bao 32(3): 385-396, 2016

Multiple on-line screening and identification methods for hydroxyl radical scavengers in Yudanshen. Journal of Pharmaceutical and Biomedical Analysis 156: 278-283, 2018

Metabonomics: evaluation of nuclear magnetic resonance (NMR) and pattern recognition technology for rapid in vivo screening of liver and kidney toxicants. Toxicological Sciences 57(2): 326-337, 2000

Saturation transfer difference nuclear magnetic resonance spectroscopy as a method for screening proteins for anesthetic binding. Molecular Pharmacology 66(4): 929-935, 2004

Rapid screening, separation, and detection of hydroxyl radical scavengers from total flavonoids of Ginkgo biloba leaves by chromatography combined with molecular devices. Journal of Separation Science 39(21): 4158-4165, 2016

Flow injection proton nuclear magnetic resonance spectroscopy combined with pattern recognition methods: Implications for rapid structural studies and high throughput biochemical screening. Analytical Communications 34(11): 339-341, 1997

Selective One-Dimensional Total Correlation Spectroscopy Nuclear Magnetic Resonance Experiments for a Rapid Identification of Minor Components in the Lipid Fraction of Milk and Dairy Products: Toward Spin Chromatography?. Journal of Agricultural and Food Chemistry 63(22): 5381-5387, 2015

Gramicidin A backbone and side chain dynamics evaluated by molecular dynamics simulations and nuclear magnetic resonance experiments. II: nuclear magnetic resonance experiments. Journal of Physical Chemistry. B 115(22): 7427-7432, 2011

Screening nucleotide binding to amino acid-coated supports by surface plasmon resonance and nuclear magnetic resonance. Analytical and Bioanalytical Chemistry 401(3): 983-993, 2011

Identification of nonpeptidic urotensin II receptor antagonists by virtual screening based on a pharmacophore model derived from structure-activity relationships and nuclear magnetic resonance studies on urotensin II. Journal of Medicinal Chemistry 45(9): 1799-1805, April 25, 2002

¹⁹F nuclear magnetic resonance screening of glucokinase activators. Analytical Biochemistry 477: 62-68, 2016