+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Screening bioactive compounds inhibiting influenza virus from isatidis radix by ultrafiltration mass spectrometry

Screening bioactive compounds inhibiting influenza virus from isatidis radix by ultrafiltration mass spectrometry

Zhongguo Zhong Yao Za Zhi 39(5): 812-816

In vitro neuraminidase inhibition assays and ultrafiltration liquid chromatography with diodearray detector coupled to time of flight mass spectrometer (UPLC-DAD-TOF-MS) were combined to screen bioactive compounds inhibiting neuraminidase from Isatidis Radix. By comparing the compounds from Isatidis Radix before and after ultrafiltration, we found that arginine, goitrin and adenosinea can bind with neuraminidase, and the binding degree of the three compounds were (36.23 +/- 1.12)%, (32.54 +/- 1.02)% and (9.38 +/- 0.47)%, respectively. The IC50 of arginine and goitrin were (1.16 +/- 0.02), (1.20 +/- 0.02) g x L(-1), respectively. While the IC50 of adenosinea was higher than 500 g x L(-1). The results showed that arginine and goitrin might be the main compounds with antiviral activity of Isatidis Radix. This study may provide a useful method for the screening of bioactive compounds and quality control of Isatidis Radix.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 055670465

Download citation: RISBibTeXText

PMID: 25204170

Related references

Antiviral activities against influenza virus (FM1) of bioactive fractions and representative compounds extracted from Banlangen (Radix Isatidis). Journal of Traditional Chinese Medicine 36(3): 369-376, 2016

Radix isatidis Polysaccharides Inhibit Influenza a Virus and Influenza A Virus-Induced Inflammation via Suppression of Host TLR3 Signaling In Vitro. Molecules 22(1), 2017

In vitro antioxidant and anti-inflammatory activities of Radix Isatidis extract and bioaccessibility of six bioactive compounds after simulated gastro-intestinal digestion. Journal of Ethnopharmacology 157(): 55-61, 2016

Screening and determination of potential xanthine oxidase inhibitors from Radix Salviae Miltiorrhizae using ultrafiltration liquid chromatography-mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 923-924: 48-53, 2013

Current advances in screening for bioactive components from medicinal plants by affinity ultrafiltration mass spectrometry. Phytochemical Analysis, 2018

Antiviral Decoction of Isatidis Radix ( bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity. Journal of Traditional and Complementary Medicine 2(1): 47-51, 2012

Evaluation of the interaction of bioactive compounds in Cortex Pseudolarix and Radix Stephaniae by the microdialysis probe coupled with high performance liquid chromatography-mass spectrometry. Journal of Chromatography. A 1216(11): 2179-2184, 2008

Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 43(1): 151-157, 2006

Development of a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method for screening mitochondria-targeted bioactive constituents from complex matrixes: Herbal medicines as a case study. Journal of Chromatography. A 1413: 33-46, 2016

Rapid screening and distribution of bioactive compounds in different parts of Berberis petiolaris using direct analysis in real time mass spectrometry. Journal of Pharmaceutical Analysis 5(5): 332-335, 2018

Discussion on the botanical origin of Isatidis radix and Isatidis folium based on DNA barcoding. Yao Xue Xue Bao 48(12): 1850-1855, 2016

Comparison of three methods of measuring indigo and indirubin in radix isatidis and folium isatidis. Journal of Northwest A and F University Natural Science Edition 35(2): 215-219, 2007

Screening of anti-endotoxin components from Radix isatidis. Journal of Huazhong University of Science and Technology. Medical Sciences 26(2): 261-264, 2006

Rapid screening of bioactive compounds from natural products by integrating 5-channel parallel chromatography coupled with on-line mass spectrometry and microplate based assays. Analytica Chimica Acta 777: 49-56, 2013

Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method. Journal of Pharmaceutical Analysis 8(4): 240-249, 2018