+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Screening compounds of Chinese medicinal herbs anti-Marek's disease virus



Screening compounds of Chinese medicinal herbs anti-Marek's disease virus



Pharmaceutical Biology 52(7): 841-847



Marek's disease (MD) seriously threatens the world poultry industry and has resulted in great economic losses. Chinese medicinal herbs are a rich source for lead compounds and drug candidates for antiviral treatments. To investigate the anti-MDV activity and mechanism of 20 compounds extracted from Chinese medicinal herbs. Antiviral assay, time of addition experiments, and virucidal assay were performed on chicken embryo fibroblast cells. The 50% cytotoxic concentration and 50% effective concentration were determined and, accordingly, selectivity index and inhibition ratio were calculated. Antiviral assay showed dipotassium glycyrrhizinate (DG) and sodium tanshinone IIA sulfonate (STS) exhibited significantly inhibitory activity against MDV in a dose-dependent manner. EC50 of DG and STS were 893.5 ± 36.99 µg/mL and 54.82 ± 2.99 µg/mL, and selective index (SI) were >3.36 and >9.12, respectively. Time of addition experiment and virucidal assay demonstrated DG inhibited viral replication in the full replication cycle and inactivated MDV particles in non-time-dependent manner, but STS interfered with the early stage of MDV replication and inactivated MDV particles in a time-dependent manner. Moreover, both DG and STS promoted apoptosis of cells infected by MDV. DG and STS have great potential for developing new anti-MDV drugs for clinic application.

(PDF emailed within 0-6 h: $19.90)

Accession: 055670560

Download citation: RISBibTeXText

PMID: 24920229

DOI: 10.3109/13880209.2013.871639


Related references

Screening of anti-simian immunodeficiency virus (SIV) drugs from Chinese medicinal herbs. Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi 13(3): 162-3, 134, 1993

Screening of anti-SIV drugs from Chinese medicinal herbs. Chinese Journal of Integrative Medicine 1(2): 134-136, 1995

Screening and characteristics of anti complementary polysaccharides from chinese medicinal herbs. Agricultural Chemistry & Biotechnology 35(1): 42-50, 1992

Screening for anti-lipase properties of 37 traditional Chinese medicinal herbs. Journal of the Chinese Medical Association 73(6): 319-324, 2010

Anti human retroviral properties of compounds purified from chinese medicinal herbs. Journal of Cellular Biochemistry Supplement (16 PART E): 86, 1992

Screening of Epstein-Barr virus early antigen expression inducers from Chinese medicinal herbs and plants. Biomedical and Environmental Sciences 7(1): 50-55, 1994

Experimental study on the anti-hepatitis B virus effect of the ordinary anti-hepatitis Chinese medicinal herbs. Zhong Xi Yi Jie He Za Zhi 9(8): 494-495, 1989

Traditional Chinese medicinal herbs as potential AChE inhibitors for anti-Alzheimer's disease: A review. Bioorganic Chemistry 75: 50-61, 2017

The Yin-Yang Property of Chinese Medicinal Herbs Relates to Chemical Composition but Not Anti-Oxidative Activity: An Illustration Using Spleen-Meridian Herbs. Frontiers in Pharmacology 9: 1304-1304, 2018

Immunoglobulins and anti-Marek's disease virus antibody synthesis in chickens after passive immunization with immunoglobulin Y anti-Marek's disease virus antibody. American Journal of Veterinary Research 36(11): 1655-1661, 1975

Epstein-Barr virus DNA polymerase inhibitors from Chinese herbs: Use of preliminary screening, physicochemical properties and taxonomy for new lead compounds generation. Chinese Pharmaceutical Journal (Taipei) 50(4): 233-247, 1998

Characterisation of new oligoglycosidic compounds in two Chinese medicinal herbs. Phytochemical Analysis 13(4): 202-206, 2002

Hepatoprotective Effects of Chinese Medicinal Herbs: A Focus on Anti-Inflammatory and Anti-Oxidative Activities. International Journal of Molecular Sciences 17(4): 465-465, 2016

Anti-fungal and anti-bacterial activities of ethanol extracts of selected traditional Chinese medicinal herbs. Asian Pacific Journal of Tropical Medicine 6(9): 673-681, 2014

Prediction Methods of Herbal Compounds in Chinese Medicinal Herbs. Molecules 23(9): -, 2018