+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Screening the life cycle of Schistosoma mansoni using high-resolution mass spectrometry



Screening the life cycle of Schistosoma mansoni using high-resolution mass spectrometry



Analytica Chimica Acta 845: 62-69



Schistosomiasis is a common tropical disease caused by Schistosoma species Schistosomiasis' pathogenesis is known to vary according to the worms' strain. Moreover, high parasitical virulence is directly related to eggs release and granulomatous inflammation in the host's organs. This virulence might be influenced by different classes of molecules, such as lipids. Therefore, better understanding of the metabolic profile of these organisms is necessary, especially for an increased potential of unraveling strain virulence mechanisms and resistance to existing treatments. In this report, direct-infusion electrospray high-resolution mass spectrometry (ESI(+)-HRMS) along with the lipidomic platform were employed to rapidly characterize and differentiate two Brazilian S. mansoni strains (BH and SE) in three stages of their life cycle: eggs, miracidia and cercariae, with samples from experimental animals (Swiss/SPF mice). Furthermore, urine samples of the infected and uninfected mice were analyzed to assess the possibility of direct diagnosis. All samples were differentiated using multivariate data analysis, PCA, which helped electing markers from distinct lipid classes; phospholipids, diacylglycerols and triacylglycerols, for example, clearly presented different intensities in some stages and strains, as well as in urine samples. This indicates that biochemical characterization of S. mansoni may help narrowing-down the investigation of new therapeutic targets according to strain composition and aggressiveness of disease. Interestingly, lipid profile of infected mice urine varies when compared to control samples, indicating that direct diagnosis of schistosomiasis from urine may be feasible.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055674480

Download citation: RISBibTeXText

PMID: 25201273

DOI: 10.1016/j.aca.2014.06.013


Related references

Characterization of plant materials by pyrolysis field ionization mass spectrometry high resolution mass spectrometry time resolved high resolution mass spectrometry and curie point pyrolysis gas chromatography mass spectrometry of spruce needles. Analytical Chemistry 61(3): 221-227, 1988

Ultra high performance liquid chromatography-time-of-flight high resolution mass spectrometry in the analysis of hexabromocyclododecane diastereomers: method development and comparative evaluation versus ultra high performance liquid chromatography coupled to Orbitrap high resolution mass spectrometry and triple quadrupole tandem mass spectrometry. Journal of Chromatography. a 1366: 73-83, 2014

Rapid screening of drugs of abuse in human urine by high-performance liquid chromatography coupled with high resolution and high mass accuracy hybrid linear ion trap-Orbitrap mass spectrometry. Journal of Chromatography. a 1302: 95, 2013

Application of real-time mass spectrometric techniques to environmental organic geochemistry. I. Computerized high resolution mass spectrometry and gas chromatography-low resolution mass spectrometry. Archives of Environmental Contamination and Toxicology 3(4): 385-409, 1975

Observations on the life-cycle of Schistosoma mansoni in the laboratory, with a discussion on the snail vectors of S. mansoni and S. haematobium. Ann Trop Med And Parasitol 41(2): 173-177, 1947

Environmental screening of acidic compounds based on capillary zone electrophoresis/laser-induced fluorescence detection with identification by gas chromatography/mass spectrometry and gas chromatography/high-resolution mass spectrometry. Journal of Aoac International 83(5): 1059-1067, 2000

Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis. International Journal of Molecular Sciences 16(7): 16085-16103, 2015

Simulation of Duty Cycle-Based Trapping and Ejection of Massive Ions Using Linear Digital Quadrupoles: the Enabling Technology for High Resolution Time-of-Flight Mass Spectrometry in the Ultra High Mass Range. International Journal of Mass Spectrometry 304(1): 36-40, 2011

Comparison of gas chromatography high resolution mass spectrometry and mass spectrometry mass spectrometry for detection of poly chlorinated bi phenyls and tetra chloro di benzo furan. Analytical Chemistry 55(4): 744-749, 1983

The application of hybrid mass spectrometry/mass spectrometry and high-resolution mass spectrometry to the analysis of fish samples for polychlorinated dibenzo-p-dioxins and dibenzofurans. Biological Mass Spectrometry 20(4): 161-168, 1991

Characterization of toxaphene by high resolution gas chromatography combined with high resolution mass spectrometry and tandem mass spectrometry. Rapid Communications In Mass Spectrometry. 11(4): 341-348, 1997

High resolution accurate mass screening of prohibited substances in equine plasma using liquid chromatography – Orbitrap mass spectrometry. Drug Testing and Analysis aop(aop), 2012

High resolution accurate mass screening of prohibited substances in equine plasma using liquid chromatography--Orbitrap mass spectrometry. Drug Testing and Analysis 5(7): 509-528, 2013

The application of high resolution and low resolution mass spectrometry in gas chromatography mass spectrometry coupling for analyzing complex volatile mixtures of plant tissue cultures. Schreier, P (Ed ) Analysis Of Volatiles: Methods And Applications; Proceedings Of The International Workshop, Wuerzburg, West Germany, Sept 28-30, 1983 Xi+469p Walter De Gruyter: Berlin, West Germany; New York, N Y , Usa Illus 307-322, 1984