+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Selectivity in capillary electrophoresis:  application to chiral separations with cyclodextrins

Selectivity in capillary electrophoresis:  application to chiral separations with cyclodextrins

Analytical Chemistry 69(3): 385-392

In order to accurately evaluate the performances of any electrolyte medium, a clear concept of selectivity in capillary electrophoresis and related electroseparation techniques is proposed. Selectivity is defined as the ratio of the affinity factors of both analytes for a separating agent (phase, pseudophase, or complexing agent present in the background electrolyte). When in the presence of a complexing agent and if only 1:1 complexation occurs, selectivity corresponds to the ratio of the apparent binding constants and is independent of the concentration of the complexing agent. This concept is illustrated through the separations of neutral and anionic enantiomers in the presence of a cationic cyclodextrin, the mono(6-amino-6-deoxy)-β-cyclodextrin, as a chiral complexing agent. The values obtained for different pairs of enantiomers are discussed with regard to the functional groups that distinguish them. When the analytes have the same mobilities in free solution and in their complexed form, then the resolution equation developed in micellar electrokinetic chromatography may be applied and optimum conditions (affinity factors, chiral agent concentration) can be predicted.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 055697856

Download citation: RISBibTeXText

PMID: 21639190

DOI: 10.1021/ac960606z

Related references

Application of sulfated cyclodextrins to chiral separations by capillary zone electrophoresis. Analytical Chemistry 68(8): 1360-1368, 1996

Chiral separations of lobeline analogs using high performance capillary electrophoresis and derivatized cyclodextrins as chiral additives. Abstracts of Papers American Chemical Society 213(1-3): MEDI 232, 1997

Rapid screening for chiral separations by short-end injection capillary electrophoresis using highly sulfated cyclodextrins as chiral selectors. Electrophoresis 22(15): 3203-3215, 2001

Chiral separations by capillary zone electrophoresis using functionalized cyclodextrins. Abstracts of Papers American Chemical Society 210(1-2): ANYL 11, 1995

Comprehensive strategy for chiral separations using sulfated cyclodextrins in capillary electrophoresis. Chirality 15(8): 709-723, 2003

Quantitative approach for the screening of cyclodextrins by nuclear magnetic resonance spectroscopy in support of chiral separations in liquid chromatography and capillary electrophoresis enantioseparation of norgestrel with alpha-, beta- and gamma-cyclodextrins. Journal of Chromatography. a 961(2): 257-276, 2002

Robustness testing of chiral separations by capillary electrophoresis using highly-sulfated cyclodextrins. Journal of Chromatography. A 1007(1-2): 165-177, 2003

Evaluation of chiral ionic liquids as additives to cyclodextrins for enantiomeric separations by capillary electrophoresis. Journal of Chromatography. a 1155(2): 134-141, 2007

Recent innovations in the use of charged cyclodextrins in capillary electrophoresis for chiral separations in pharmaceutical analysis. Electrophoresis 21(15): 3220-3239, 2000

Modeling and optimization of the chiral selectivity of basic analytes in chiral capillary electrophoresis with negatively charged cyclodextrins using electrochemical detection. Electrophoresis 22(10): 2025-2031, 2001

Systematic screening approach for chiral separations of basic compounds by capillary electrophoresis with modified cyclodextrins. Journal of Pharmaceutical and Biomedical Analysis 19(5): 679-694, 1999

Knowledge-based system for method development of chiral separations with capillary electrophoresis using highly-sulphated cyclodextrins. Journal of Pharmaceutical & Biomedical Analysis 27(3-4): 515-529, 15 January, 2002

Separation selectivity in chiral and achiral capillary electrophoresis with mixed cyclodextrins. Journal of Chromatography A 792(1-2): 297-307, 1997

Chiral separations of underivatized arylpropionic acids by capillary zone electrophoresis with various cyclodextrins: acidity and inclusion constant determinations. Journal of Chromatography A 735(1-2): 311-320, 1996

Optimization of enantiomeric separations for basic compounds in capillary zone electrophoresis using neutral and anionic cyclodextrins as chiral selectors. Biomedical Chromatography 9(6): 265-266, 1995