EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Sequence-based typing--molecular typing of Legionella pneumophila strains within the framework of an international external quality assessment


Sequence-based typing--molecular typing of Legionella pneumophila strains within the framework of an international external quality assessment



Medycyna Doswiadczalna i Mikrobiologia 65(2): 103-110



ISSN/ISBN: 0025-8601

PMID: 24180137

The aim of the study was evaluation results of molecular typing L. pneumophila strains that was carried by using SBT (Sequence-Based Typing) method, obtained by laboratory of Department of Bacteriology NIZP-PZH within the framework of the ninth international external quality assessment (ELDSNet Legionella pneumophila DNA SBT) and their comparision with the results obtained by other reference laboratories in EU. The panel of five coded isolates of L. pneumophila was investigated. Genomic DNA of Legionella were extracted and defined regions of seven genes were amplified by PCR and sequenced. Then, consensus sequence of the correct length were generated. In order to determine the complete allelic profile and Seqence Type (ST) forward and reverse sequence for each allele were submitted online by using the L. pneumophila database. All of L. pneumoniae isolates sent to genotyping by SBT method were correctly identified in our laboratory. Results of the ninth international external quality assessment have confirmed competences of laboratory of Department of Bacteriology NIZP-PZH in typing of L. pneumophila isolates accordance with the requirements of the international classification.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 055719852

Download citation: RISBibTeXText

Related references

External quality assessment of a DNA sequence-based scheme for epidemiological typing of Legionella pneumophila by an international network of laboratories. Journal of Clinical Microbiology 45(10): 3251-3256, 2007

Molecular typing of Legionella pneumophila isolates from environmental water samples and clinical samples using a five-gene sequence typing and standard Sequence-Based Typing. Plos one 13(2): E0190986, 2018

The N-acylneuraminate cytidyltransferase gene, neuA, is heterogenous in Legionella pneumophila strains but can be used as a marker for epidemiological typing in the consensus sequence-based typing scheme. Journal of Clinical Microbiology 49(12): 4052-4058, 2011

A combined molecular typing approach does not discriminate Legionella pneumophila serogroup 1 strains of a predominant sequence-based type in Palermo, Italy. Journal of Infection and Public Health 2(4): 184-188, 2009

Sequence-based typing of Legionella pneumophila strains isolated from hospital water distribution systems as a complementary element of risk assessment of legionellosis in Poland. Annals of Agricultural and Environmental Medicine: Aaem 20(3): 436-440, 2013

Sequence-based typing of 82 strains of serotype i Legionella pneumophila isolated from 9 provinces in China. Zhonghua Yu Fang Yi Xue Za Zhi 45(10): 890-894, 2011

Extension of the Legionella pneumophila sequence-based typing scheme to include strains carrying a variant of the N-acylneuraminate cytidylyltransferase gene. Clinical Microbiology and Infection: the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases 20(7): O435-O441, 2014

Does better identification of the Legionella pneumophila serogroup 1 strains by Sequence-Based Typing (SBT) allow for the implementation of more effective contamination control strategies and more targeted intervention measures?. Microchemical Journal 115: 126-129, 2014

Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR. Journal of Clinical Microbiology 34(6): 1595-1598, 1996

Molecular typing of Legionella pneumophila strains isolated from environment in Morocco. Cellular and Molecular Biology 58 Suppl: Ol1709-Ol1714, 2012

Addition of neuA, the gene encoding N-acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains. Journal of Clinical Microbiology 45(6): 1965-1968, 2007

Molecular typing of Legionella pneumophila serogroup 1 clinical strains isolated in Italy. International Journal of Medical Microbiology: Ijmm 304(5-6): 597-602, 2014

Genotypic diversity of Legionella pneumophila in environmental and clinical strains assessed by Sequence-Based Typing, in association with retrospective clinical surveillance in Northern Italy. Annals of agricultural and environmental medicine: AAEM 23(2): 248-253, 2016

Sequence-based typing of enviromental Legionella pneumophila isolates in Guangzhou. Wei Sheng Wu Xue Bao 51(3): 320-325, 2011

Molecular genetic typing of Legionella pneumophila serogroup 1 isolated in town Verkhnyaya Pyshma using international standards. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2008(2): 20-23, 2008