+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Serial analysis of gene expression adapted for downsized extracts (SAGE/SADE) analysis in reticulocytes



Serial analysis of gene expression adapted for downsized extracts (SAGE/SADE) analysis in reticulocytes



Methods in Molecular Biology 496: 299-311



Reticulocytes are the last stage of erythropoiesis before red blood cells (RBC). Although most of the RBC proteins have been characterized, little is known about expression profile of their mRNA during differentiation. Our goal was to initiate a genomic global solution to provide a transcriptional data base on which it will be possible to follow the erythroid differentiation and study RBC disorders. We used a modified protocol of serial analysis of gene expression (SAGE), the serial analysis of downsized extracts (SADE) which allows identification of genes from small amounts of mRNA and is thus an appropriate method starting from reticulocyte. SAGE does not depend on previous knowledge of genes. The method produces a set of cellular transcribed gene signatures (tags) and provides a comprehensive view of cells phenotypes by computational analyses.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 055722342

Download citation: RISBibTeXText

PMID: 18839119


Related references

Differential gene expression in premalignant human epidermis revealed by cluster analysis of serial analysis of gene expression (SAGE) libraries. Faseb Journal 16(2): 246-248, 2002

Genomic and gene expression analysis of Lentinula edodes and Coptinopsis cinerea by Serial Analysis of Gene Expression (SAGE). 2007

Gene expression profiling of human GV oocytes: An analysis of a profile obtained by Serial Analysis of Gene Expression (SAGE). Journal of Reproductive Immunology 53(1-2): 193-201, 2002

Serial analysis of gene expression (SAGE): experimental method and data analysis. Current Protocols in Human Genetics Chapter 11: Unit 11.7, 2007

Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags. Nucleic Acids Research 27(17): E17, 1999

USAGE: a web-based approach towards the analysis of SAGE data. Serial Analysis of Gene Expression. Bioinformatics 16(10): 899-905, 2000

Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE). Parasitology 136(5): 469-485, 2009

Serial analysis of gene expression (SAGE): Advances, analysis and applications to pigment cell research. Pigment Cell Research 16(3): 183-189, 2003

A comparative molecular analysis of developing mouse forelimbs and hindlimbs using serial analysis of gene expression (SAGE). Genome Research 11(10): 1686-1698, 2001

SAGE. The serial analysis of gene expression. Methods in Molecular Biology 99: 297-319, 2000

Serial analysis of gene expression (SAGE). Methods in Molecular Biology 383: 41-66, 2007

Serial analysis of gene expression (SAGE). 2007

Reverse serial analysis of gene expression (SAGE) characterization of orphan SAGE tags from human embryonic stem cells identifies the presence of novel transcripts and antisense transcription of key pluripotency genes. Stem Cells 24(5): 1162-1173, 2006

Comparison of oligonucleotide-microarray and serial analysis of gene expression (SAGE) in transcript profiling analysis of megakaryocytes derived from CD34+ cells. Experimental & Molecular Medicine 35(5): 460-466, 2003

Comparative transcriptome analysis of in vivo- and in vitro-produced porcine blastocysts by small amplified RNA-serial analysis of gene expression (SAR-SAGE). Molecular Reproduction and Development 75(6): 976-988, 2008