+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Serotonin uptake is largely mediated by platelets versus lymphocytes in peripheral blood cells



Serotonin uptake is largely mediated by platelets versus lymphocytes in peripheral blood cells



Acs Chemical Neuroscience 4(1): 161-170



The serotonin transporter (SERT), a primary target for many antidepressants, is expressed in the brain and also in peripheral blood cells. Although platelet SERT function is well accepted, lymphocyte SERT function has not been definitively characterized. Due to their small size, platelets often are found in peripheral blood mononuclear cell preparations aimed at isolating lymphocytes, monocytes, and macrophages. The presence of different cells makes it difficult to assign SERT expression and function to specific cell types. Here, we use flow cytometry and IDT307, a monoamine transporter substrate that fluoresces after uptake into cells, to investigate SERT function in lymphocyte and platelet populations independently, as well as simultaneously without prior isolation. We find that murine lymphocytes exhibit temperature-dependent IDT307 transport but uptake is independent of SERT. Lack of measurable SERT function in lymphocytes was corroborated by chronoamperometry using serotonin as a substrate. When we examined rhesus and human mixed blood cell populations, we found that platelets, and not lymphocytes, were primary contributors to SERT function. Overall, these findings indicate that lymphocyte SERT function is minimal. Moreover, flow cytometry, in conjunction with the fluorescent transporter substrate IDT307, can be widely applied to investigate SERT in platelets from populations of clinical significance.

(PDF emailed within 0-6 h: $19.90)

Accession: 055727094

Download citation: RISBibTeXText

PMID: 23336055

DOI: 10.1021/cn300146w


Related references

Initial evidence for serotonin uptake by human peripheral blood lymphocytes. Biogenic Amines 3(3): 193-200, 1985

The stereoselectivity of serotonin uptake in brain tissue and blood platelets: the topography of the serotonin uptake area. Neuroscience and Biobehavioral Reviews 10(1): 37-46, 1986

Serotonin mediated increases in cyclic gmp levels and their relation to serotonin uptake in human platelets an indicator of serotonergic function. Barchas, J D And W E Bunney, Jr (Ed ) Neurology And Neurobiology (New York), Vol 40 Perspectives in Psychopharmacology: A Collection Of Papers in Honor Of Earl Usdin; Neuroscience Research Symposium, Irvine, California, Usa, 1984 Xxxvii+681p Alan R Liss, Inc : new York, New York, Usa Illus 365-374, 1988

Decreased uptake of 3H-serotonin and endogenous content of serotonin in blood platelets in hypertensive patients. Hypertension 6(4): 568-573, 1984

Effect of doxepin on serotonin metabolism in rat brain and serotonin uptake by human blood platelets. Progress in Neuro-Psychopharmacology 4(3): 253-260, 1980

Uptake of serotonin and 1-methyl-4-phenylpyridinum into human platelets and lymphocytes. Naunyn-Schmiedeberg's Archives of Pharmacology 357(4 SUPPL): R30, 1998

Genetic variation in the serotonin transporter promoter region affects serotonin uptake in human blood platelets. American Journal of Medical Genetics 88(1): 83-87, Feb 5, 1999

Decreased uptake of tritium labeled serotonin and endogenous content of serotonin in blood platelets in hypertensive patients. Hypertension 6(4): 568-573, 1984

Correlation between serotonin uptake in human blood platelets with the 44-bp polymorphism and the 17-bp variable number of tandem repeat of the serotonin transporter. American Journal of Medical Genetics 114(3): 323-328, April 8, 2002

Assessment of blood platelets as a model for CNS response: comparative effects of caffeine on 5-HT uptake and release mechanisms in rat platelets and rat brain serotonin neurons. Life Sciences 33(12): 1149-1156, 1983

Interaction of choriocarcinoma cells and human peripheral blood lymphocytes. Resistance of cultured choriocarcinoma cells to cell-mediated cytotoxicity by mitogen-activated lymphocytes. Journal of Clinical Investigation 63(3): 428-436, 1979

Adriamycin-mediated potentiation of cytotoxicity against freshly isolated bladder cancer cells by autologous non-activated peripheral blood lymphocytes and tumor infiltrating lymphocytes. Journal of Urology 162(6): 2170-2175, 1999

Uptake of L-leucine by trout red blood cells and peripheral lymphocytes. Journal of Membrane Biology 152(1): 57-63, 1996

Normal serotonin uptake by blood platelets and brain synaptosomes but selective impairment of platelet serotonin storage in mice with Chediack-Higashi syndrome. Life Sciences 38(24): 2193-2198, 1986

Effects of serotonin uptake inhibitor lilly 110140 3 p tri fluoromethylphenoxy n methyl 3 phenyl propylamine hydro chloride on transport of serotonin in rat and human blood platelets. Biochemical Pharmacology 25(7): 865-867, 1976