EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis



Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis



Analyst 137(13): 3099-3101



We present a design for a sheath-flow cuvette that uses a relatively inexpensive quartz cuvette. The cuvette has a high optical quality square flow chamber that is fused to quartz tubes at each end. PEEK/TEFZEL fittings hold and seal the quartz flow chamber without putting strain on the cuvette. The performance of the cuvette is evaluated as a laser-induced fluorescence detector for capillary electrophoresis. The cuvette produces mass detection limits of 50 yoctomoles (30 copies) for 5-carboxyl tetramethylrhodamine (5 TAMRA SE) with a separation efficiency of 400,000 theoretical plates.

(PDF emailed within 0-6 h: $19.90)

Accession: 055782757

Download citation: RISBibTeXText

PMID: 22606689

DOI: 10.1039/c2an35321k



Related references

Capillary gel electrophoresis for DNA sequencing. Laser-induced fluorescence detection with the sheath flow cuvette. Journal of Chromatography 516(1): 61-67, 1990

Sheath-flow cuvette for high-sensitivity laser-induced fluorescence detection in capillary electrophoresis. Applied Spectroscopy 61(7): 777-779, 2007

Fiber optic illumination of a poly(dimethylsiloxane) sheath flow cuvette for diode laser induced fluorescence detection in capillary electrophoresis. Electrophoresis 36(4): 502-508, 2015

Ultrasensitive near-infrared laser-induced fluorescence detection in capillary electrophoresis using a diode laser and avalanche photodiode. Journal of Chromatography A 779(1-2): 185-194, 1997

Evaluation of a sheath flow cuvette for postcolumn fluorescence derivatization of DNA fragments separated by capillary electrophoresis. Analytical Chemistry 70(1): 182-186, 1998

Design of 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde as a reagent for ultrasensitive determination of primary amines by capillary electrophoresis using laser fluorescence detection. Analytical Chemistry 63(5): 408-412, 1991

Ultrasensitive on-column laser-induced fluorescence in capillary electrophoresis using multiparameter confocal detection. Analyst 137(23): 5538-5545, 2013

Ultrasensitive detection of closely related angiotensin I peptides using capillary electrophoresis with near-infrared laser-induced fluorescence detection. Analytical Chemistry 71(3): 667-671, 1999

Six orders of magnitude dynamic range in capillary electrophoresis with ultrasensitive laser-induced fluorescence detection. Talanta 80(2): 744-748, 2010

An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection. Electrophoresis 26(12): 2384-2390, 2005

Ultrasensitive detection of genetically modified maize DNA by capillary gel electrophoresis with laser-induced fluorescence using different fluorescent intercalating dyes. Journal of agricultural and food chemistry1, 50(16): 4497-4502, 2002

Ultrasensitive detection of indoleamines by combination of nanoparticle-based extraction with capillary electrophoresis/laser-induced native fluorescence. Journal of Chromatography. A 1216(36): 6451-6458, 2009

Post-capillary laser-induced fluorescence detection without sheath-flow. Journal of Chromatography A 844(1-2): 433-438, June 4, 1999

Rapid Detection of Adenovirus in Fecal Samples by Capillary Electrophoresis-laser Induced Fluorescence and Microchip Capillary Electrophoresis-laser Induced Fluorescence. Sichuan Da Xue Xue Bao. Yi Xue Ban 46(4): 615-8, 627, 2016

Ultrasensitive analysis of lysergic acid diethylamide and its C-8 isomer in hair by capillary zone electrophoresis in combination with a stacking technique and laser induced fluorescence detection. Analytica Chimica Acta 866: 90-98, 2015