Simultaneous determination of cyadox and its metabolites in plasma by high-performance liquid chromatography tandem mass spectrometry

He, L.; Liu, K.; Su, Y.; Zhang, J.; Liu, Y.; Zeng, Z.; Fang, B.; Zhang, G.

Journal of Separation Science 34(15): 1755-1762


ISSN/ISBN: 1615-9314
PMID: 21721120
DOI: 10.1002/jssc.201000822
Accession: 055787759

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Cyadox is a novel antimicrobial growth-promoter of the quinoxalines. For food safety and pharmacokinetic studies, a convenient, sensitive and reproducible LC-ESI-MS/MS method was developed for the simultaneous determination of cyadox and its major metabolites, quinoxaline-2-carboxylic acid, 1,4-bisdesoxycyadox, cyadox-1-monoxide and cyadox-4-monoxide in chicken plasma. Plasma sample was subjected to a simple deproteinisation with acetonitrile. Analysis was performed on a C18 column by detection with mass spectrometry in multiple reaction monitoring mode. A gradient elution program with 0.2% formic acid, methanol and acetonitrile was performed at a flow rate of 0.2 mL/min. The decision limits (CCαs) of five analytes in plasma ranged from 1.0 to 4.0 μg/L, and the detection capabilities (CCβs) were <10 μg/L. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The extraction recoveries of five analytes were between 87.4 and 93.9% in plasma at the spiked levels of 5 (10)-200 μg/L with the relative standard deviations <10% for each analyte. The developed method demonstrated a satisfactory applicability in real plasma samples.