EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Simultaneous isocratic separation of phenolic acids and flavonoids using micellar liquid chromatography


Simultaneous isocratic separation of phenolic acids and flavonoids using micellar liquid chromatography



Journal of Separation Science 36(23): 3667-3672



ISSN/ISBN: 1615-9306

PMID: 24106139

DOI: 10.1002/jssc.201300920

The simultaneous isocratic separation of a mixture of five phenolic acids and four flavonoids (two important groups of natural polyphenolic compounds with very different polarities) was investigated in three different RPLC modes using a hydro-organic mobile phase, and mobile phases containing SDS at concentrations below and above the critical micellar concentration (submicellar LC and micellar LC (MLC), respectively). In the hydro-organic mode, methanol and acetonitrile; in the submicellar mode methanol; and in the micellar mode, methanol and 1-propanol were examined individually as organic modifiers. Regarding the other modes, MLC provided more appropriate resolutions and analysis time and was preferred for the separation of the selected compounds. Optimization of separation in MLC was performed using an interpretative approach for each alcohol. In this way, the retention of phenolic acids and flavonoids were modeled using the retention factors obtained from five different mobile phases, then the Pareto optimality method was applied to find the best compatibility between analysis time and quality of separation. The results of this study showed some promising advantages of MLC for the simultaneous separation of phenolic acids and flavonoids, including low consumption of organic solvent, good resolution, short analysis time, and no requirement of gradient elution.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055789777

Download citation: RISBibTeXText

Related references

Simultaneous quantification of flavonoids and phenolic acids in plant materials by a newly developed isocratic high-performance liquid chromatography approach. Journal of Agricultural and Food Chemistry 58(20): 10812-10816, 2010

Improvement of the sensitivity of two-dimensional liquid chromatography-micellar electrokinetic capillary chromatography separation of phenolic acids and flavonoids natural antioxidants using the on-line preconcentration step. 2012

Separation of phenolic acids and flavone natural antioxidants by two-dimensional method combining liquid chromatography and micellar electrokinetic capillary chromatography. Electrophoresis 31(13): 2200-2210, 2010

Simultaneous optimization of surfactant concentration and organic modifier in micellar liquid chromatography. Application to the separation of phenolic compounds. Analytical Letters 33(8): 1691-1709, 2000

Separation of some phenolic acids in micellar liquid chromatography using design of experiment-response surface methodology. Journal of Analytical Chemistry 71(6): 610-616, 2016

High performance liquid chromatography separation and wavelength area ratios of more than 50 phenolic acids and flavonoids. Journal of Chemical Ecology 11(3): 383-396, 1985

Simultaneous determination of 49 amino acids, B vitamins, flavonoids, and phenolic acids in commonly consumed vegetables by ultra-performance liquid chromatography-tandem mass spectrometry. Food Chemistry 2020: 128712, 2020

Simultaneous quantification of flavonoids and phenolic acids in Herba Scutellariae barbatae and its confused plants by high performance liquid chromatography-tandem mass spectrometry. Food Chemistry 129(3): 1297-1304, 2011

Simultaneous determination of main phenolic acids and flavonoids in tomato by micellar electrokinetic capillary electrophoresis. Food Chemistry 221: 439-446, 2017

Determination of phenolic acids in fruit juices by isocratic column liquid chromatography. Journal of Chromatography A 891(1): 183-188, 2000

Simultaneous detection of flavonoids and phenolic acids in Herba Lysimachiae and Herba Desmodii Styracifolii using liquid chromatography tandem mass spectrometry. Food Chemistry 138(1): 139-147, 2013

Quantitative analysis of flavonoids and phenolic acids in Arnica montana L. by micellar electrokinetic capillary chromatography. Analytica Chimica Acta 614(2): 196-200, 2008

The application of solid-phase extraction and reversed phase high-performance liquid chromatography for simultaneous isolation and determination of plant flavonoids and phenolic acids. Chromatographia 57(Suppl): S77-S80, 2003

Separation of PTH-amino acids by isocratic high-performance liquid chromatography. Journal of Chromatography 154(2): 313-317, 1978

Simultaneous determination of mycophenolic acid and its phenolic glucuronide in human plasma using an isocratic high-performance liquid chromatography procedure. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 817(2): 327-330, 2005