EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Single domain antibody templated nanoparticle resistors for sensing



Single domain antibody templated nanoparticle resistors for sensing



Biosensors & Bioelectronics 25(8): 1908-1913



The diversity of biomolecules enables materials to be synthesized at ambient conditions into complex shapes and structures while affording improved control over nanoparticle synthesis, structures, properties, and functionality. Also, biomolecules can impart recognition and sensing capabilities to metal nanoparticles. Here, llama single domain antibodies selected towards the beta-subunit of cholera toxin were used to template the synthesis of gold nanoparticles and subsequently assembled onto electrodes via dielectrophoresis. These assemblies were then tested for the detection of cholera toxin via changes in the conductance of antibody coated gold nanoparticles.

(PDF same-day service: $19.90)

Accession: 055793703

Download citation: RISBibTeXText

PMID: 20117926

DOI: 10.1016/j.bios.2010.01.004



Related references

Comprehensive optimization of a single-chain variable domain antibody fragment as a targeting ligand for a cytotoxic nanoparticle. Mabs 7(1): 42-52, 2015

Single domain antibody-alkaline phosphatase fusion proteins for antigen detection--analysis of affinity and thermal stability of single domain antibody. Journal of Immunological Methods 393(1-2): 1-7, 2013

Multiplex single-nucleotide polymorphism typing by nanoparticle-coupled DNA-templated reactions. Journal of the American Chemical Society 131(33): 11668-9, 2009

Self-templated synthesis of single-crystal and single-domain ferroelectric nanoplates. Angewandte Chemie 51(37): 9283-9287, 2012

Templated synthesis of single-walled carbon nanotube and metal nanoparticle assemblies in solution. Journal of the American Chemical Society 128(47): 15078-9, 2006

Chain stiffness regulates entropy-templated perfect mixing at single-nanoparticle level. Nanoscale 8(2): 1024-1032, 2015

Photostable single-molecule nanoparticle optical biosensors for real-time sensing of single cytokine molecules and their binding reactions. Journal of the American Chemical Society 130(50): 17095-17105, 2009

An antibody single-domain phage display library of a native heavy chain variable region: isolation of functional single-domain VH molecules with a unique interface. Journal of Molecular Biology 290(3): 685-698, 1999

Construction of expression vectors and study on single-chain antibody and reshaping single-domain antibody against CD3. Science in China. Series C, Life Sciences 40(3): 270-276, 1997

Thermoluminescence apparatus using PT100 resistors as the heating and sensing elements. Review of Scientific Instruments 78(8): 083905-083905, 2007

A capacitive-pulse model for nanoparticle sensing by single conical nanochannels. Nanoscale 8(3): 1565-1571, 2015

Capillary Flow Resistors: Local and Global Resistors. Langmuir 32(3): 915-921, 2015

Accelerometers and force sensing resistors for optimal control of walking of a hemiplegic. IEEE Transactions on Bio-Medical Engineering 55(8): 1973-1984, 2008

Determining level of postural control in young adults using force-sensing resistors. IEEE Transactions on Information Technology in Biomedicine 15(4): 608-614, 2011