+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Spontaneous formation of stably-attached and 3D-organized hepatocyte aggregates on oxygen-permeable polydimethylsiloxane membranes having 3D microstructures



Spontaneous formation of stably-attached and 3D-organized hepatocyte aggregates on oxygen-permeable polydimethylsiloxane membranes having 3D microstructures



Biomedical Microdevices 12(3): 465-475



In order to enhance the viability and the differentiated functions of primary hepatocytes in cultures, it appears important to have them organized within a three-dimensional (3D) structure which promotes extensive cell-cell contacts, but also to be adequately supplied with oxygen. Here, we report a simple methodology satisfying these two fundamental but sometimes conflicting issues: primary rat hepatocytes were cultured on polydimethylsiloxane (PDMS) membranes with 3D-pillared microstructures with various dimensions, so that the cells could organize themselves around the pillars into various kinds of 3D multicellular aggregates, while being continuously supplied with oxygen by diffusion through the PDMS membrane. As expected, under such conditions, hepatocyte cultures exhibited higher albumin secretion and urea synthesis rates. It appeared then that as the spacing decreased between the pillars, the cells were more stably organized into smaller spherical aggregates and displayed the highest albumin secretion rates. Such a simple design is likely to be included in a new drug/chemical screening system in a practical microplate format, but also appears applicable to microfluidic devices.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055889310

Download citation: RISBibTeXText

PMID: 20174871

DOI: 10.1007/s10544-010-9403-8


Related references

Microfibrillated cellulose sheets coating oxygen-permeable PDMS membranes induce rat hepatocytes 3D aggregation into stably-attached 3D hemispheroids. Journal of Biomaterials Science. Polymer Edition 22(11): 1509-1522, 2011

Spontaneous formation of highly functional three-dimensional multilayer from human hepatoma Hep G2 cells cultured on an oxygen-permeable polydimethylsiloxane membrane. Tissue Engineering. Part C Methods 16(2): 311-318, 2010

Direct oxygen supply with polydimethylsiloxane (PDMS) membranes induces a spontaneous organization of thick heterogeneous liver tissues from rat fetal liver cells in vitro. Cell Transplantation 21(2-3): 401-410, 2012

Participation of intracellular membranes in the formation of high permeable domains in plasma membranes of epithelial cells during vasopressin stimulation of water transport. Tsitologiya 33(11): 135-140, 1991

Phenotypes of non-attached Pseudomonas aeruginosa aggregates resemble surface attached biofilm. Plos one 6(11): E27943, 2011

A sponge matrix method for tissue culture; formation of organized aggregates of cells in vitro. Journal of the National Cancer Institute 12(3): 545-561, 1951

Non-local models for the formation of hepatocyte-stellate cell aggregates. Journal of Theoretical Biology 267(1): 106-120, 2010

Gas-permeable membranes and co-culture with fibroblasts enable high-density hepatocyte culture as multilayered liver tissues. Biotechnology Progress 27(4): 1146-1153, 2011

Molding of deep polydimethylsiloxane microstructures for microfluidics and biological applications. Journal of Biomechanical Engineering 121(1): 28-34, 1999

Rapid prototyping of microstructures in polydimethylsiloxane (PDMS) by direct UV-lithography. Lab on a Chip 11(7): 1368-1371, 2011

Magnetophoretic manipulation in microsystem using carbonyl iron-polydimethylsiloxane microstructures. Biomicrofluidics 8(5): 054103, 2014

The importance of physiological oxygen concentrations in the sandwich cultures of rat hepatocytes on gas-permeable membranes. Biotechnology Progress 30(6): 1401-1410, 2015

Colloid lithography-induced polydimethylsiloxane microstructures and their application to cell patterning. Biotechnology Letters 28(3): 169-173, 2006

Formation of semi-permeable membranes from cellulose acetates. Polymer Science U.S.S.R. 15(5): 1320-1325, 1973