+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Spontaneous in vitro transformation of primary human osteoblast-like cells



Spontaneous in vitro transformation of primary human osteoblast-like cells



Cancer Genomics and Proteomics 7(2): 61-66



Two new tumor-like cell lines were established which developed spontaneously in vitro from normal human primary osteoblast-like cells originating from non-oncogenic bone surgery. The tumor cell properties studied included morphology, proliferation characteristics in normal and low-serum media, and anchorage-independent growth in soft agarose. Karyotyping of the cells showed numerous rearrangements and abnormalities. These results pointed to the tumorigenic potential of the cells and demonstrate the importance of biosafety in tissue engineering and therapeutic cell applications when prolonged culture conditions are required.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 055889700

Download citation: RISBibTeXText

PMID: 20335519


Related references

The effect of platelet-rich plasma in vitro on primary cells: rat osteoblast-like cells and human endothelial cells. Tissue Engineering. Part a 16(10): 3159-3172, 2010

Changes in hla a b c expression during spontaneous transformation of human urothelial cells in vitro. European Journal of Cancer & Clinical Oncology 23(7): 991-996, 1987

Changes in HLA, A,B,C expression during spontaneous transformation of human urothelial cells in vitro. European Journal of Cancer and Clinical Oncology 23(7): 991-995, 1987

Effect of 1alpha,25(OH)2-vitamin D3 on TNF alpha-mediated apoptosis of human primary osteoblast-like cells in vitro. Hormone and Metabolic Research 31(12): 653-656, 2000

Effect of oestrogen and 1alpha, 25 2-vitamin D3 on the activity and growth of human primary osteoblast-like cells in vitro. Human Reproduction 16(Abstract Book 1): 195, 2001

Effect of estrogen and 1alpha,25(OH)2- vitamin D3 on the activity and growth of human primary osteoblast-like cells in vitro. Fertility and Sterility 77(5): 1038-1043, 2002

Pitfalls in spontaneous in vitro transformation of human mesenchymal stem cells. Experimental Cell Research 316(9): 1648-1650, 2010

Development of the osteoblast phenotype in primary human osteoblasts in culture: Comparison with rat calvarial cells in osteoblast differentiation. Journal of Cellular Biochemistry 75(1): 22-35, 1999

In vitro response of primary rat osteoblasts to titania/hydroxyapatite coatings compared with transformed human osteoblast-like cells. Journal of Materials Science. Materials in Medicine 13(8): 797-801, 2002

Comment to: "Spontaneous transformation of adult mesenchymal stem cells from cynomolgus macaques in vitro" by Z. Ren et al. Exp. Cell Res. 317 (2011) 2950-2957: spontaneous transformation of mesenchymal stem cells in culture: facts or fiction?. Experimental Cell Research 318(5): 441-443, 2012

On spontaneous in vitro transformation of human cells. II. Fetal heart-lung-fibroblasts. Archiv für Geschwulstforschung 28(3): 213-233, 1966

Spontaneous malignant transformation of human ovarian surface epithelial cells in vitro. Clinical Cancer Research 7(12): 4280-4287, 2001

Creatine supplementation stimulates collagen type I and osteoprotegerin secretion of healthy and osoteopenic primary human osteoblast-like cells in vitro. Bone 42(supp-S1): 0, 2008

In vitro response of primary human bone marrow stromal cells to recombinant human bone morphogenic protein-2 in the early and late stages of osteoblast differentiation. Development Growth and Differentiation 50(7): 553-564, 2008

Progesterone receptors are expressed in human osteoblast-like cell lines and in primary human osteoblast cultures. Calcified Tissue International 57(6): 436-441, 1995