+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging



Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging



Optics Letters 33(2): 113-115



We demonstrate stimulated emission depletion (STED) microscopy implemented in a laser scanning confocal microscope using excitation light derived from supercontinuum generation in a microstructured optical fiber. Images with resolution improvement beyond the far-field diffraction limit in both the lateral and axial directions were acquired by scanning overlapped excitation and depletion beams in two dimensions using the flying spot scanner of a commercially available laser scanning confocal microscope. The spatial properties of the depletion beam were controlled holographically using a programmable spatial light modulator, which can rapidly change between different STED imaging modes and also compensate for aberrations in the optical path. STED fluorescence lifetime imaging microscopy is demonstrated through the use of time-correlated single photon counting.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055926993

Download citation: RISBibTeXText

PMID: 18197209

DOI: 10.1364/OL.33.000113


Related references

Supercontinuum stimulated emission depletion fluorescence lifetime imaging. Journal of Physical Chemistry. B 116(27): 7821-7826, 2012

A 3D aligning method for stimulated emission depletion microscopy using fluorescence lifetime distribution. Microscopy Research and Technique 77(11): 935-940, 2014

Dual-mode super-resolution imaging with stimulated emission depletion microscopy and fluorescence emission difference microscopy. Applied Optics 54(17): 5425-5431, 2015

An Excitation Emission Fluorescence Lifetime Spectrometer (EEFLS) using a frequency doubled supercontinuum laser source. Methods and Applications in Fluorescence, 2018

Supercontinuum source tuned by an on-axis monochromator for fluorescence lifetime imaging. Optics Express 18(19): 20505-20511, 2011

Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source. Optics Letters 32(23): 3408-3410, 2007

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics Letters 19(11): 780-782, 1994

Picosecond polarized supercontinuum generation controlled by intermodal four-wave mixing for fluorescence lifetime imaging microscopy. Optics Express 16(23): 18844-9, 2009

Stimulated-emission-depletion microscopy with a multicolor stimulated-Raman-scattering light source. Optics Letters 33(21): 2491-2493, 2008

Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source. Journal of Microscopy 225(Pt 2): 126-136, 2007

Long-distance fluorescence lifetime imaging using stimulated emission. Journal of Biomedical Optics 17(1): 011009-011009, 2012

Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission. Optics Express 20(27): 28216-28221, 2013

Combined non-linear laser imaging (two-photon excitation fluorescence microscopy, fluorescence lifetime imaging microscopy, multispectral multiphoton microscopy) in cutaneous tumours: first experiences. Journal of the European Academy of Dermatology and Venereology 23(3): 314-316, 2009

Long working distance fluorescence lifetime imaging with stimulated emission and electronic time delay. Optics Express 20(10): 11445-11450, 2012

Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging. Biomedical Optics Express 3(6): 1465-1475, 2012