+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Strains of Mycobacterium tuberculosis from western Maharashtra, India, exhibit a high degree of diversity and strain-specific associations with drug resistance, cavitary disease, and treatment failure

Strains of Mycobacterium tuberculosis from western Maharashtra, India, exhibit a high degree of diversity and strain-specific associations with drug resistance, cavitary disease, and treatment failure

Journal of Clinical Microbiology 48(10): 3593-3599

We performed spoligotyping of Mycobacterium tuberculosis isolates from 833 systematically sampled pulmonary tuberculosis (TB) patients in urban Mumbai, India (723 patients), and adjacent rural areas in western India (110 patients). The urban cohort consisted of two groups of patients, new cases (646 patients) and first-time treatment failures (77 patients), while only new cases were recruited in the rural areas. The isolates from urban new cases showed 71% clustering, with 168 Manu1, 62 CAS, 22 Beijing, and 30 EAI-5 isolates. The isolates from first-time treatment failures were 69% clustered, with 14 Manu1, 8 CAS, 8 Beijing, and 6 EAI-5 isolates. The proportion of Beijing strains was higher in this group than in urban new cases (odds ratio [OR], 3.29; 95% confidence limit [95% CL], 1.29 to 8.14; P = 0.003). The isolates from rural new cases showed 69% clustering, with 38 Manu1, 7 CAS, and 1 EAI-5 isolate. Beijing was absent in the rural cohort. Manu1 was found to be more common in the rural cohort (OR, 0.67; 95% CL, 0.42 to 1.05; P = 0.06). In total, 71% of isolates were clustered into 58 spoligotypes with 4 predominant strains, Manu1 (26%), CAS (9%), EAI-5 (4%), and Beijing (4%), along with 246 unique spoligotypes. In the isolates from urban new cases, we found Beijing to be associated with multidrug resistance (MDR) (OR, 3.40; 95% CL, 1.20 to 9.62; P = 0.02). CAS was found to be associated with pansensitivity (OR, 1.83; 95% CL, 1.03 to 3.24; P = 0.03) and cavities as seen on chest radiographs (OR, 2.72; 95% CL, 1.34 to 5.53; P = 0.006). We recorded 239 new spoligotypes yet unreported in the global databases, suggesting that the local TB strains exhibit a high degree of diversity.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 055932085

Download citation: RISBibTeXText

PMID: 20720028

DOI: 10.1128/jcm.00430-10

Related references

Whole genome sequencing of clinical strains of Mycobacterium tuberculosis from Mumbai, India: A potential tool for determining drug-resistance and strain lineage. Tuberculosis 107: 63-72, 2017

Mycobacterium tuberculosis strains exhibit differential and strain-specific molecular signatures in pulmonary epithelial cells. Developmental and Comparative Immunology 65: 321-329, 2016

On the lability of the degree of drug resistance of Mycobacterium tuberculosis strains. Antibiotiki 12(8): 718-722, 1967

Molecular diversity of drug resistant mycobacterium tuberculosis strains in Western Turkey. Balkan Medical Journal 29(2): 160-165, 2012

Genetic diversity of Mycobacterium tuberculosis in Peru and exploration of phylogenetic associations with drug resistance. Plos one 8(6): E65873, 2013

Strain diversity, epistasis and the evolution of drug resistance in Mycobacterium tuberculosis. Clinical Microbiology and Infection 17(6): 815-820, 2011

Drug resistance and Mycobacterium tuberculosis strain diversity in TB/HIV co-infected patients in Ho Chi Minh city, Vietnam. Journal of Global Antimicrobial Resistance 10: 154-160, 2017

A study of Mycobacterium tuberculosis genotypic diversity & drug resistance mutations in Varanasi, north India. Indian Journal of Medical Research 139(6): 892-902, 2014

Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil. Brazilian Journal of Infectious Diseases 17(3): 338-345, 2013

Is the Beijing strain of Mycobacterium tuberculosis associated with cavitary lung disease?. Infection Genetics and Evolution 33: 1-5, 2015

Association of mutations in rpsL gene with high degree of streptomycin resistance in clinical isolates of Mycobacterium tuberculosis in India. Indian Journal of Medical Research 129(1): 108-110, 2009

Genome-wide analysis of Mycobacterium tuberculosis polymorphisms reveals lineage-specific associations with drug resistance. Bmc Genomics 20(1): 252, 2019

Primary and acquired drug resistance in Mycobacterium tuberculosis strains in western region of Libyan Arab Jamahiriya. Tropical and Geographical Medicine 41(4): 304-308, 1989

Resistance, of Mycobacterium tuberculosis to isonicotinic acid hydrazide in the treatment of cavitary pulmonary tuberculosis. Boletin Cultural e Informativo - Consejo General de Colegios Medicos de Espana 12(62): 43-46, 1952

Clonal diversity and drug resistance in Mycobacterium tuberculosis isolated from extra-pulmonary samples in central India--a pilot study. Indian Journal of Medical Microbiology 32(4): 434-437, 2015