+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Stress response of transgenic tobacco plants expressing a cyanobacterial ferredoxin in chloroplasts



Stress response of transgenic tobacco plants expressing a cyanobacterial ferredoxin in chloroplasts



Plant Molecular Biology 76(6): 535-544



Expression of the chloroplast electron shuttle ferredoxin is induced by light through mechanisms that partially depend on sequences lying in the coding region of the gene, complicating its manipulation by promoter engineering. Ferredoxin expression is also down-regulated under virtually all stress situations, and it is unclear if light-dependent induction and stress-dependent repression proceed through the same or similar mechanisms. Previous reports have shown that expression of a cyanobacterial flavodoxin in tobacco plastids results in plants with enhanced tolerance to adverse environmental conditions such as drought, chilling and xenobiotics (Tognetti et al. in Plant Cell 18:2035-2050, 2006). The protective effect of flavodoxin was linked to functional replacement of ferredoxin, suggesting the possibility that tolerant phenotypes might be obtained by simply increasing ferredoxin contents. To bypass endogenous regulatory constraints, we transformed tobacco plants with a ferredoxin gene from Anabaena sp. PCC7120, which has only 53% identity with plant orthologs. The cyanobacterial protein was able to interact in vitro with ferredoxin-dependent plant enzymes and to mediate NADP(+) photoreduction by tobacco thylakoids. Expression of Anabaena ferredoxin was constitutive and light-independent. However, homozygous lines accumulating threefold higher ferredoxin levels than the wild-type failed to show enhanced tolerance to oxidative stress and chilling temperatures. Under these adverse conditions, Anabaena ferredoxin was down-regulated even faster than the endogenous counterparts. The results indicate that: (1) light- and stress-dependent regulations of ferredoxin expression proceed through different pathways, and (2) overexpression of ferredoxin is not an alternative to flavodoxin expression for the development of increased stress tolerance in plants.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055939596

Download citation: RISBibTeXText

PMID: 21584860

DOI: 10.1007/s11103-011-9786-9


Related references

Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts. Plant Cell Reports 26(5): 591-598, 2007

Cyanobacterial flavodoxin complements ferredoxin deficiency in knocked-down transgenic tobacco plants. Plant Journal 65(6): 922-935, 2011

Evaluation of the defense system in chloroplasts to photooxidative stress caused by paraquat using transgenic tobacco plants expressing catalase from Escherichia coli. Plant and Cell Physiology 41(3): 311-320, 2000

Enhanced tolerances of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologen-mediated oxidative stress. Plant cell and environment 25(7): 873-882, 2002

Construction and analysis of transgenic plants of Nicotiana tabacum L. expressing the bacterial gene for beta -1,3-glucanase. II. Transgenic tobacco plants expressing the bacterial gene for beta -1,3-glucanase from Clostridium thermocellum - a model for the study of differential expression of stress response genes. Genetika Moskva 32(2): 204-210, 1996

Construction and analysis of transgenic plants of Nicotiana tabacum L. expressing a bacterial gene for beta-1,3-glucanase. II. Transgenic tobacco plants expressing the bacterial beta-glucanase gene from Clostridium thermocellum,--a model for studying the differential expression of stress response-related genes. Genetika 32(2): 204-210, 1996

Response of the antioxidative system in chloroplasts of transgenic tobacco plants overexpressing thylakoid-membrane-bound ascorbate peroxidase under photooxidative stress. Plant & Cell Physiology 42(Suppl.): s179, 2001

Transgenic tobacco plants expressing antisense ferredoxin-NADP(H) reductase transcripts display increased susceptibility to photo-oxidative damage. Plant Journal 35(3): 332-341, 2003

Nitrogen fixation persists under conditions of salt stress in transgenic Medicago truncatula plants expressing a cyanobacterial flavodoxin. Plant Biotechnology Journal 8(9): 954-965, 2011

Osmotic Stress Tolerance of Transgenic Tobacco Expressing a Gene Encoding a Membrane-Located Receptor-like Protein from Tobacco Plants. Plant Physiology 131(2): 454-462, 2003

Osmotic stress tolerance of transgenic tobacco expressing a gene encoding a membrane-located receptor-like protein from tobacco plants. Plant Physiology 131(2): 454-462, 2003

Influence of Environmental Stress on Biomass Partitioning in Transgenic Tobacco Plants Expressing the Movement Protein of Tobacco Mosaic Virus. Plant Physiology 114(2): 475-481, 1997

Enhanced tolerance of transgenic potato plants expressing choline oxidase in chloroplasts against water stress. Botanical Studies 54(1): 30, 2013

Transgenic tobacco plants with an improved tolerance towards oxidative stress in chloroplasts. Photosynthesis: from light to biosphere Volume IV Proceedings of the Xth International Photosynthesis Congress, Montpellier, France, 20-25 August 1995: 165-170, 1995

Biomass partitioning and carbon allocation in transgenic tobacco plants expressing the movement protein of tobacco mosaic virus as influenced by environmental stress factors. Journal of Experimental Botany 47(SPEC ISSUE): 1318, 1996