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Structural basis for the autoinhibition of the C-terminal kinase domain of human RSK1

Li, D.; Fu, T.M.; Nan, J.; Liu, C.; Li, L.F.; Su, X.D.

Acta Crystallographica. Section D Biological Crystallography 68(Part 6): 680-685

2012


ISSN/ISBN: 1399-0047
PMID: 22683790
DOI: 10.1107/s0907444912007457
Accession: 055948699

P90 ribosomal S6 kinases (RSKs) respond to various mitogen stimuli and comprise two distinct protein kinase domains. The C-terminal kinase domain (CTKD) receives signal from ERK1/2 and adopts an autoinhibitory mechanism. Here, the crystal structure of human RSK1 CTKD is reported at 2.7 Å resolution. The structure shows a standard kinase fold, with the catalytic residues in the ATP-binding cleft orientated in optimal conformations for phosphotransfer. The inactivation of the CTKD is conferred by an extra α-helix (αL), which occupies the substrate-binding groove. In combination with previous knowledge, this structure indicates that activation of RSK1 involves the removal of αL from the substrate-binding groove induced by ERK1/2 phosphorylation.

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