+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Subinhibitory concentrations of triclosan promote Streptococcus mutans biofilm formation and adherence to oral epithelial cells



Subinhibitory concentrations of triclosan promote Streptococcus mutans biofilm formation and adherence to oral epithelial cells



Plos one 9(2): E89059



Triclosan is a general membrane-active agent with a broad-spectrum antimicrobial activity that is commonly used in oral care products. In this study, we investigated the effect of sub-minimum inhibitory concentrations (MICs) of triclosan on the capacity of the cariogenic bacterium Streptococcus mutans to form biofilm and adhere to oral epithelial cells. As quantified by crystal violet staining, biofilm formation by two reference strains of S. mutans was dose-dependently promoted, in the range of 2.2- to 6.2-fold, by 1/2 and 1/4 MIC of triclosan. Observations by scanning electron microscopy revealed the presence of a dense biofilm attached to the polystyrene surface. Growth of S. mutans in the presence of triclosan at sub-MICs also increased its capacity to adhere to a monolayer of gingival epithelial cells. The expression of several genes involved in adherence and biofilm formation in S. mutans was investigated by quantitative RT-PCR. It was found that sub-MICs of triclosan significantly increased the expression of comD, gtfC, and luxS, and to a lesser extent of gtfB and atlA genes. These findings stress the importance of maintaining effective bactericidal concentrations of therapeutic triclosan since sub-MICs may promote colonization of the oral cavity by S. mutans.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 055987728

Download citation: RISBibTeXText

PMID: 24551218


Related references

In vitro inhibition of Streptococcus mutans biofilm formation on hydroxyapatite by subinhibitory concentrations of anthraquinones. Antimicrobial Agents and ChemoTherapy 51(4): 1541-1544, 2007

The effect of subinhibitory concentrations of gentian violet on the germ tube formation by Candida albicans and its adherence to oral epithelial cells. Archives of Oral Biology 82: 1-5, 2017

Effects of subinhibitory concentrations of chemical agents on hydrophobicity and in vitro adherence of Streptococcus mutans and Streptococcus sanguis. Caries Research 28(5): 335-341, 1994

Influence of subinhibitory concentrations of antibiotics on aggregation and adherence of streptococcus mutans. Adam, D , H Hahn And W Opferkuch (Ed ) The Influence Of Antibiotics on The Host-Parasite Relationship Ii Second International Symposium, Munich, West Germany, Mar 28-30, Xiv+298p Springer-Verlag: Berlin, West Germany; New York, N Y , Usa Illus 209-219, 1985

Fibrinogen-induced streptococcus mutans biofilm formation and adherence to endothelial cells. Biomed Research International 2013: 431465, 2013

Dual role for pilus in adherence to epithelial cells and biofilm formation in Streptococcus agalactiae. Plos Pathogens 5(5): E1000422, 2009

Study of the effects of chitosan upon Streptococcus mutans adherence and biofilm formation. Anaerobe 20: 27-31, 2013

The effect of c-di-GMP (3'-5'-cyclic diguanylic acid) on the biofilm formation and adherence of Streptococcus mutans. Microbiological Research 165(2): 87-96, 2010

Inhibition of Streptococcus mutans adherence and biofilm formation using analogues of the SspB peptide. Archives of Oral Biology 55(10): 754-762, 2010

Subinhibitory Concentrations of Amoxicillin, Lincomycin, and Oxytetracycline Commonly Used to Treat Swine Increase Streptococcus suis Biofilm Formation. Frontiers in Microbiology 9: 2707, 2018

Effect of high-fructose corn syrup on the acidogenicity, adherence and biofilm formation of Streptococcus mutans. Australian Dental Journal 58(2): 213-218, 2013

Streptococcus mutans adherence and biofilm formation on experimental composites containing dicalcium phosphate dihydrate nanoparticles. Journal of Materials Science. Materials in Medicine 28(7): 108, 2017

Effects of Nidus Vespae extract and chemical fractions on glucosyltransferases, adherence and biofilm formation of Streptococcus mutans. Archives of Oral Biology 52(9): 869-875, 2007

Reduction of Streptococcus mutans adherence and dental biofilm formation by surface treatment with phosphorylated polyethylene glycol. Antimicrobial Agents and ChemoTherapy 51(10): 3634-3641, 2007

Streptococcal adhesin SspA/B analogue peptide inhibits adherence and impacts biofilm formation of Streptococcus mutans. Plos one 12(4): E0175483, 2017