+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Surface imprinted thin polymer film systems with selective recognition for bovine serum albumin



Surface imprinted thin polymer film systems with selective recognition for bovine serum albumin



Analytica Chimica Acta 718: 109-115



Molecularly imprinted polymers are synthetic antibody mimics formed by the crosslinking of organic or inorganic polymers in the presence of an analyte which yields recognitive polymer networks with specific binding pockets for that biomolecule. Surface imprinted polymers were synthesized via a novel technique for the specific recognition of bovine serum albumin (BSA). Thin films of recognitive networks based on 2-(dimethylamino)ethyl methacrylate (DMAEMA) as the functional monomer and varying amounts of either N,N'-methylenebisacrylamide (MBA) or poly(ethylene glycol) (400) dimethacrylate (PEG400DMA) as the crosslinking agent were synthesized via UV free-radical polymerization and characterized. A clear and reproducible increase in recognition of the template BSA was demonstrated for these systems at 1.6-2.5 times more BSA recognized by the MIP sample relative to the control polymers. Additionally, these polymers exhibited selective recognition of the template relative to competing proteins with up to 2.9 times more BSA adsorbed than either glucose oxidase or bovine hemoglobin. These synthetic antibody mimics hold significant promise as the next generation of robust recognition elements in a wide range of bioassay and biosensor applications.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 056023359

Download citation: RISBibTeXText

PMID: 22305905

DOI: 10.1016/j.aca.2012.01.006


Related references

Bovine serum albumin surface imprinted polymer fabricated by surface grafting copolymerization on zinc oxide rods and its application for protein recognition. Journal of Separation Science 38(19): 3477-3486, 2016

Preparation and characterization of bovine serum albumin surface-imprinted thermosensitive magnetic polymer microsphere and its application for protein recognition. Biosensors and Bioelectronics 51: 261-267, 2014

Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein. Biosensors and Bioelectronics 31(1): 84-89, 2012

m-Aminophenylboronic Acid as a Functional Monomer for Fabricating Molecularly Imprinted Polymer for the Recognition of Bovine Serum Albumin. Reactive and Functional Polymers 68(9): 1291-1296, 2008

Preparation of molecular imprinted polymer with quaternary ammonium groups as recognition sites for separation of pig cyclophilin 18 and bovine serum albumin. Journal of Separation Science 33(12): 1856-1862, 2010

Hierarchical thin film architectures for enhanced sensor performance: liquid crystal-mediated electrochemical synthesis of nanostructured imprinted polymer films for the selective recognition of bupivacaine. Biosensors 4(2): 90-110, 2015

Preparation and adsorption characteristics of surface-imprinted polymer using bovine serum albumin and lysozyme as double template proteins. Se Pu 33(5): 475-480, 2015

Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin. Biosensors and Bioelectronics 54: 266-272, 2014

Multiwalled carbon nanotubes-ceramic electrode modified with substrate-selective imprinted polymer for ultra-trace detection of bovine serum albumin. Biosensors and Bioelectronics 39(1): 236-243, 2013

The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation. Journal of Chromatography. a 1218(22): 3489-3495, 2011

Molecularly imprinted polymer film grafted from porous silica for selective recognition of testosterone. Analytical Letters 39(2): 275-286, 2006

Selective histamine piezoelectric chemosensor using a recognition film of the molecularly imprinted polymer of bis(bithiophene) derivatives. Analytical Chemistry 81(7): 2633-2643, 2009

Surface plasmon resonance sensor for profenofos detection using molecularly imprinted thin film as recognition element. Food Control 25(2): 543-549, 2012

Molecularly imprinted thin film self-assembled on piezoelectric quartz crystal surface by the sol-gel process for protein recognition. Biosensors and Bioelectronics 21(7): 1244-1251, 2005

Surface imprinted beads for the recognition of human serum albumin. Biosensors and Bioelectronics 22(9-10): 2322-2328, 2007