+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Synovium fragment-derived cells exhibit characteristics similar to those of dissociated multipotent cells in synovial fluid of the temporomandibular joint

Synovium fragment-derived cells exhibit characteristics similar to those of dissociated multipotent cells in synovial fluid of the temporomandibular joint

Plos one 9(7): E101896

Multipotent mesenchymal stem cells (MSCs) found in the synovial fluid (SFMSCs) of the tempromandibular joint (TMJ) remain poorly understood. During TMJ arthrocentesis, we discovered that synovial fluid collected from some patients with TMJ disorders contained not only SFMSCs but also synovium fragments (SFs). In this study, we attempted to characterize both the SFMSCs and SF-derived cells (SFCs) in order to further understand the role of MSCs in the synovial fluid of the TMJ. The SFs were membranous and translucent and consisted of several cell layers, indicating that their origin was only from the intima. SFCs were obtained by digestion of the SFs and subsequently expanded in vitro. SFMSCs were enriched by centrifugation of the synovial fluid and expanded in vitro. SFCs and SFMSCs displayed a similar fibroblast-like, spindle-shaped morphology, and we observed that some SFMSCs grew out of small tissue masses in culture. Flow cytometric analysis showed that both groups of cells expressed similar surface markers, including CD90, CD44, CD105, and CD73. However, both were negative for Stro-1, CD146, CD45, CD34, CD11b, CD19, and HLA-DR. Immunofluorescent staining showed that both SFs and SFMSCs expressed vascular cell adhesion molecule 1. Both SFCs and SFMSCs could be induced to differentiate down osteogenic, chondrogenic, adipogenic, and neurogenic lineages in vitro. Together, our results indicate that the intima is the most likely tissue origin of SFMSCs in the TMJ. Moreover, the SFs are composed of only intima and thus offer an improved source of synovium-derived MSCs compared to synovium specimens obtained by surgery, which contain both intima and subintima.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 056062048

Download citation: RISBibTeXText

PMID: 25003199

DOI: 10.1371/journal.pone.0101896

Related references

Synovial fluid‑derived synovial fragments represent an improved source of synovial mesenchymal stem cells in the temporomandibular joint. International Journal of Molecular Medicine 41(1): 173-183, 2018

Characteristics and usefulness of synovial fluid-derived stem cells compared with synovium-derived stem cells. Osteoarthritis and Cartilage 26: S23-S24, 2018

Pluripotency of mesenchymal cells derived from synovial fluid in patients with temporomandibular joint disorder. Life Sciences 89(19-20): 741-747, 2011

Synovial Mesenchymal Stem Cells Derived From the Cotyloid Fossa Synovium Have Higher Self-renewal and Differentiation Potential Than Those From the Paralabral Synovium in the Hip Joint. American Journal of Sports Medicine 46(12): 2942-2953, 2018

Analysis of factors related to the number of mesenchymal stem cells derived from synovial fluid of the temporomandibular joint. Zhonghua Kou Qiang Yi Xue Za Zhi 52(6): 355-359, 2017

Role of synovium-derived fibrous cartilage in temporomandibular joint synovial chondromatosis. Journal of Oral Pathology and Medicine 48(1): 79-86, 2019

From osteoarthritic synovium to synovial-derived cells characterization: synovial macrophages are key effector cells. Arthritis Research and Therapy 18: 83, 2016

HDAC10 upregulation contributes to interleukin 1β-mediated inflammatory activation of synovium-derived mesenchymal stem cells in temporomandibular joint. Journal of Cellular Physiology 234(8): 12646-12662, 2019

Synovial chondromatosis of the temporomandibular joint: the effect of interleukin-1 on loose-body-derived cells. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics 85(5): 526-531, 1998

IL-1β impedes the chondrogenic differentiation of synovial fluid mesenchymal stem cells in the human temporomandibular joint. International Journal of Molecular Medicine 39(2): 317-326, 2017

Induction of osteoclast-like cells derived from the synovial lavage fluids of patients with temporomandibular joint disorders. Osteoarthritis and Cartilage 15(3): 291-299, 2007

Properties of rheumatoid and normal synovial tissue in vitro and cells derived from them. Production of prostaglandins and collagenase in response to factors derived from cultured blood mononuclear cells and from synovium. Rheumatology International 2(3): 113-120, 1982

Higher chondrogenic potential of fibrous synovium- and adipose synovium-derived cells compared with subcutaneous fat-derived cells: distinguishing properties of mesenchymal stem cells in humans. Arthritis and Rheumatism 54(3): 843-853, 2006

The pilot study of fibrin with temporomandibular joint derived synovial stem cells in repairing TMJ disc perforation. Biomed Research International 2014: 454021, 2014

Human amniotic fluid-derived stem cells have characteristics of multipotent stem cells. Cell Proliferation 40(1): 75-90, 2007