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Systemic identification of estrogen-regulated genes in breast cancer cells through cap analysis of gene expression mapping



Systemic identification of estrogen-regulated genes in breast cancer cells through cap analysis of gene expression mapping



Biochemical and Biophysical Research Communications 447(3): 531-536



To explore the estrogen-regulated genes genome-widely in breast cancer, cap analysis of gene expression (CAGE) sequencing was performed in MCF-7 cells under estrogen treatment. Estrogen-regulated expressional changes were found in 1537 CAGE tag clusters (TCs) (⩾1.5 or ⩽0.66-folds). Among them, 15 TCs were situated in the vicinity of (⩽10 kb) reported estrogen receptor-binding sites. Knockdown experiments of the 15 TC-associated genes demonstrated that the genes such as RAMP3, ISOC1 and GPRC5C potentially regulate the growth or migration of MCF-7 cells. These results suggest that CAGE sequencing will reveal novel estrogen target genes in breast cancer.

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Accession: 056095125

Download citation: RISBibTeXText

PMID: 24746470

DOI: 10.1016/j.bbrc.2014.04.033


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