+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Constitutive expression of CmSKOR, an outward K + channel gene from melon, in Arabidopsis thaliana involved in saline tolerance

Constitutive expression of CmSKOR, an outward K + channel gene from melon, in Arabidopsis thaliana involved in saline tolerance

Plant Science 274: 492-502

Shaker-like K+ outward rectifying channel (SKOR) is involved in mediating long-distance K+ transport from roots to shoots. In this study, a Shaker-like outward K+ channel gene CmSKOR (GenBank accession number MF447462) was isolated from melon (Cucumis melo L.). Phylogenetic analysis showed that CmSKOR belongs to the SKOR-subfamily in the Shaker-like K+ channel family. Electrophysiological experiments indicated that CmSKOR was a K+-permeable channel with low affinity. Expressed in Xenopus oocytes, CmSKOR displayed classical Shaker-like outwardly rectifying K+ currents. Confocal imaging of a CmSKOR - yellow fluorescent fusion protein (YFP) in transgenic Nicotiana tabacum leaves indicated that CmSKOR was located in the plasma membrane. Transcript analysis showed CmSKOR predominantly expressed in melon roots and with lower abundance in stem and leaves. In addition, both external K+ and NaCl treatment could up-regulate the expression of CmSKOR in melon and enhance the K+ content in shoot. Constitutive overexpressed CmSKOR in Arabidopsis thaliana, the transgenic plants showed changes in root length in MS plates, displayed higher maximum photochemical efficiency of PSII (Fv/Fm), higher fresh and dry weight, and accumulation of K+ in shoot, together with the changes of transcript amount of CmSKOR with NaCl treatments in mixture substrate. In conclusion, it was proposed that CmSKOR may play the role on distributing K+ to the shoot in melon and its constitutive expression in Arabidopsis improved saline tolerance by maintaining K+ homeostasis in the plant.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 056177356

Download citation: RISBibTeXText

PMID: 30080639

DOI: 10.1016/j.plantsci.2018.07.005

Related references

Effect of constitutive expression of the COR15a gene on the freezing tolerance of isolated protoplasts of Arabidopsis thaliana. Cryobiology 33(6): 656-657, 1996

Constitutive expression of the pea ABA-responsive 17 (ABR17) cDNA confers multiple stress tolerance in Arabidopsis thaliana. Plant Biotechnology Journal 4(5): 529-549, 2006

Isolation and gene expression analysis of Arabidopsis thaliana mutants with constitutive expression of ATL2, an early elicitor-response RING-H2 zinc-finger gene. Genetics 167(2): 919-929, 2004

Semi-constitutive expression of an Arabidopsis thaliana alpha-tubulin gene. Plant Molecular Biology 21(5): 937-942, 1993

CYP709B3, a cytochrome P450 monooxygenase gene involved in salt tolerance in Arabidopsis thaliana. Bmc Plant Biology 13: 169, 2013

Induction of RAB18 gene expression and activation of K+ outward rectifying channels depend on an extracellular perception of ABA in Arabidopsis thaliana suspension cells. Plant journal: for cell and molecular biology 18(1): 13-22, 1999

Characterization of the Chloride Channel-Like, AtCLCg, Involved in Chloride Tolerance in Arabidopsis thaliana. Plant and Cell Physiology 57(4): 764-775, 2016

Constitutive non-inducible expression of the Arabidopsis thaliana Nia 2 gene in two nitrate reductase mutants of Nicotiana plumbaginifolia. Plant molecular biology 33(6): 953-964, 1997

Constitutive expression of the SAP1 gene from willow (Salix discolor) causes early flowering in Arabidopsis thaliana. Development Genes and Evolution 216(1): 19-28, 2006

GEK1, a gene product of Arabidopsis thaliana involved in ethanol tolerance, is a D-aminoacyl-tRNA deacylase. Nucleic Acids Research 35(3): 930-938, 2007

Identification of ICE2, a gene involved in cold acclimation which determines freezing tolerance in Arabidopsis thaliana. Gene429(1-2):98, 2009

Transformation of tobacco with an Arabidopsis thaliana gene involved in trehalose biosynthesis increases tolerance to several abiotic stresses. Euphytica 146(1/2): 165-176, 2005

Expression of BjMT2, a metallothionein 2 from Brassica juncea, increases copper and cadmium tolerance in Escherichia coli and Arabidopsis thaliana, but inhibits root elongation in Arabidopsis thaliana seedlings. Journal of Experimental Botany 57(14): 3575-3582, 2006

Regulatory sequences involved in the peroxidase gene expression in Arabidopsis thaliana. Plant Cell Reports 13(3-4): 123-129, 1994

A transient outward-rectifying K+ channel current down-regulated by cytosolic Ca2+ in Arabidopsis thaliana guard cells. Proceedings of the National Academy of Sciences of the United States of America 95(11): 6548-6553, 1998