+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The Lan3-2 glycoepitope of Hirudo medicinalis consists of beta-(1,4)-linked mannopyranose: cell type-specific glycans of H. medicinalis



The Lan3-2 glycoepitope of Hirudo medicinalis consists of beta-(1,4)-linked mannopyranose: cell type-specific glycans of H. medicinalis



Journal of Neurochemistry 107(5): 1448-1456



While glycosyltransferases are restrictively expressed in invertebrate model organisms, little is known of their glycan end products. One such restrictively expressed glycoepitope was localized to sensory and epithelial cells of leech and Caenorhabditis elegans using the Lan3-2 monoclonal antibody. A biological function for the neural Lan3-2 epitope was previously determined in the leech. Here we report on the chemical structure of this mannosidic epitope harvested from whole Hirudo medicinalis. Crude glycans were liberated from glycoproteins by hydrazinolysis. Re-N-acetylated glycans were subjected to immunoaffinity purification. The affinity-purified glycans were fractioned by size chromatography into oligosaccharides and polysaccharides. Lan3-2 oligosaccharide structure was characterized by gas chromatography of alditol acetates, methylation analysis, 500 MHz 1H NMR spectroscopy, matrix-assisted laser desorption/ionization mass spectrometry, and electrospray ionization tandem MS-MS of permethylated derivatives. The predominant components of the Lan3-2 oligosaccharide fraction were a series of linear beta-(1,4)-linked mannose polymers. The homologous expression of the Lan3-2 epitope in C. elegans will facilitate the exploration of its glycosylation pathway. Other invertebrates expressing the Lan3-2 epitope are Planaria dugesia, Capitella sp. I and Lumbriculus variegatus. The glycoepitope was not detected in the diploblastic animals Hydra littoralis and Aptaisia sp. or in deuterostomes.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 056195707

Download citation: RISBibTeXText

PMID: 19013832


Related references

The Lan3-2 glycoepitope of Hirudo medicinalis consists of -(1,4)-linked mannopyranose. 2008

Separation and characterization of cell type-specific poly-beta- -linked mannopyranose from the leech species Hirudo medicinalis. Glycobiology 12(10): 679, 2002

New stations of Hirudo medicinalis in the Szczecin Lowland Nowe stanowiska pijawki lekarskiej Hirudo medicinalis na Nizinie Szczecinskiej. Chronmy Przyrode Ojczysta. Listopad-Grudzien; 556: 111-112, 1999

The medicinal leech Hirudo medicinalis found in Western Jutland Laegeiglen Hirudo medicinalis fundet i Vestjylland. Flora og Fauna 108(4): 101-102, 2002

Basic studies on Hirudo medicinalis for a space experiment. II. Behavior of Hirudo medicinalis in unnatural environments. Space Life Sciences 2(1): 45-47, 1970

Basic studies on Hirudo medicinalis for a space experiment. I. Behavior of Hirudo medicinalis in natural environment. Space Life Sciences 2(1): 40-44, 1970

Does the medicinal leech Hirudo medicinalis feel well in The Netherlands Hirudinea? Voelt de medicinale bloedzuiger Hirudo medicinalis zich wel zo lekker in Nederland Hirudinea?. Nederlandse Faunistische Mededelingen 12: 1-10, 2000

Phylogenetic conservation of the cell-type-specific Lan3-2 glycoepitope in Caenorhabditis elegans. Development Genes and Evolution 220(3-4): 77-87, 2010

Breeding and growing of medical leeches under laboratory conditions part 1 breeding of hirudo medicinalis f serpentina and hirudo medicinalis f officinalis. Liet Tsr Moksiu Akad Darb Ser B (3): 71-76, 1972

First isolation of a giant virus from wild Hirudo medicinalis leech: Mimiviridae isolation in Hirudo medicinalis. Viruses 5(12): 2920-2930, 2013

A new station of Hirudo medicinalis Linnaeus, 1758 in central Poland Nowe stanowisko pijawki lekarskiej Hirudo medicinalis Linnaeus, 1758 w srodkowej Polsce. Chronmy Przyrode Ojczysta. Marzec-Kwiecien; 622: 103-105, 2006

Breeding and growing of medicinal leeches under laboratory conditions 1 Breeding of Hirudo medicinalis f serpentina and H medicinalis f officinalis. Trudy Akad Nauk Litovskoi SSR (Ser C), 59: 71-76, 1972

Cell- and tissue-specific expression of putative protein kinase mRNAs in the embryonic leech, Hirudo medicinalis. Cell and Tissue Research 280(3): 479-489, 1995

A Kazal-type inhibitor of human mast cell tryptase: isolation from the medical leech Hirudo medicinalis, characterization, and sequence analysis. Biological Chemistry Hoppe-Seyler 375(10): 685-694, 1994

Morphogenesis of segment specific innervation patterns in an identified leech hirudo medicinalis neuron. Society for Neuroscience Abstracts 11(2): 956, 1985