The effect of layer-by-layer chitosan-hyaluronic acid coating on graft-to-bone healing of a poly (ethylene terephthalate) artificial ligament
Li, H.; Ge, Y.; Zhang, P.; Wu, L.; Chen, S.
Journal of Biomaterials Science. Polymer Edition 23(1-4): 425-438
ISSN/ISBN: 1568-5624 PMID: 21255485 DOI: 10.1163/092050610x551989
Surface coating with an organic layer-by-layer self-assembled template of chitosan and hyaluronic acid on a poly(ethylene terephthalate) (PET) artificial ligament was designed for the promotion and enhancement of graft-to-bone healing after artificial ligament implantation in a bone tunnel. The results of in vitro culturing of MC3T3-E1 mouse osteoblastic cells supported the hypothesis that the layer-by-layer coating of chitosan and hyaluronic acid could promote the cell compatibility of grafts and could promote osteoblast proliferation. A rabbit extra-articular tendon-to-bone healing model was used to evaluate the effect of this kind of surface-modified stainless artificial ligament in vivo. The final results proved that this organic compound coating could significantly promote and enhance new bone formation at the graft-bone interface histologically and, correspondingly, the experimental group with coating had significantly higher biomechanical properties compared with controls at 8 weeks (P < 0.05).